BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Exported	522,457	4 bp→CC	coding (2536‑2539/2592 nt) coding (385‑388/462 nt)	BCHEFKBB_00548 → BCHEFKBB_00549 ←	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	522,457	0	T	.	100.0%	511.3 / NA	101	coding (2536/2592 nt) coding (388/462 nt)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (49/52); total (49/52)
*	Exported	522,458	0	A	.	99.0%	503.6 / ‑1.3	101	coding (2537/2592 nt) coding (387/462 nt)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (49/51); minor base G (0/1); total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-02
*	Exported	522,459	0	T	C	100.0%	385.8 / NA	101	Y846Y (TAT→TAC) Y129R (TAT→CGT)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (49/52); total (49/52)
*	Exported	522,460	0	A	C	100.0%	388.3 / NA	101	M847L (ATG→CTG) Y129R (TAT→CGT)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (49/52); total (49/52)

Only 100 of 101 total aligned reads displayed.

AACTGGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCC‑G‑C‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAA  >  Exported/522338‑522579

||

aaCTGGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCCGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGAtttt                                                                                            >  2:762969/1‑151 (MQ=255)

  cTGGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGAtttt                                                                                            >  1:531728/1‑149 (MQ=255)

  cTGGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGAtttt                                                                                            >  1:806485/1‑149 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCCAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:566594/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAATAGCAGAGTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:1564848/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  1:973152/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:1511716/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:842883/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:202993/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  1:1379786/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  1:975739/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  1:503789/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:230384/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  2:254974/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  1:229183/1‑150 (MQ=255)

    ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                         >  1:975740/1‑150 (MQ=255)

     gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                        <  1:94371/150‑1 (MQ=255)

      aTTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGa                                                                                      >  2:319760/1‑151 (MQ=255)

      aTTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGa                                                                                      >  1:318929/1‑151 (MQ=255)

            ggCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGa                                                                                >  1:124363/1‑151 (MQ=255)

              cTTGTCGGCAGCACAATCCATCCGACAACATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAACCACGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTAGCTCTTCGGCGAGATTTTCTACGATTCGGata                                                                              <  2:1219691/151‑1 (MQ=255)

              cTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCACTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGata                                                                              <  2:256787/151‑1 (MQ=255)

              cTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGATCACCGG‑‑CCTGCC‑G‑CCGGCTCTCTCTTCGGCGAGATTTTCTACGATTCGCata                                                                              >  1:1452861/1‑151 (MQ=255)

               ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                             <  2:1381435/151‑1 (MQ=255)

               ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                             <  2:1381452/151‑1 (MQ=255)

               ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                             <  2:1381507/151‑1 (MQ=255)

               ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                             <  2:1381510/151‑1 (MQ=255)

                 gTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGAGTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGAGATAg                                                                           >  2:224740/1‑151 (MQ=255)

                  tCGGCAGCACACTCCATCCGACCCAAGTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                          <  2:1312775/151‑1 (MQ=255)

                  tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTTCGATTCGGCTATAGa                                                                          <  2:1312773/151‑1 (MQ=255)

                  tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                           <  1:928231/150‑1 (MQ=255)

                  tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                           <  1:412905/150‑1 (MQ=255)

                  tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                          <  1:1163387/151‑1 (MQ=255)

                  tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                          <  1:692578/151‑1 (MQ=255)

                  tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                          <  2:84188/151‑1 (MQ=255)

                   cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                         <  1:894960/151‑1 (MQ=255)

                   cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                         <  2:1378316/151‑1 (MQ=255)

                   cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                         <  2:1058831/151‑1 (MQ=255)

                   cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                         <  2:636102/151‑1 (MQ=255)

                       agcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATg                                                                     >  1:1190370/1‑151 (MQ=255)

                        gcccaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAGGCAGAGTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                    >  2:202633/5‑151 (MQ=255)

                        gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                    >  1:1117345/1‑151 (MQ=255)

                        gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                    >  1:1217626/1‑151 (MQ=255)

                        gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATGTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                    >  1:1217627/1‑151 (MQ=255)

                         caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                   <  1:959399/151‑1 (MQ=255)

                         caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                   <  2:445476/151‑1 (MQ=255)

                         caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                   <  2:1206804/151‑1 (MQ=255)

                           aaaTCCATCCGACCAACTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                 <  2:405198/151‑1 (MQ=255)

                           aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                 >  2:787565/1‑151 (MQ=255)

                           aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                 >  2:29593/1‑151 (MQ=255)

                           aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                 >  2:1298221/1‑151 (MQ=255)

                           aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                 >  1:983959/1‑151 (MQ=255)

                           aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                 >  1:884109/1‑151 (MQ=255)

                                    cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                        <  2:80647/119‑1 (MQ=255)

                                    cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                        >  1:80678/1‑119 (MQ=255)

                                      aCCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaac                                                       <  1:28918/150‑1 (MQ=255)

                                         aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                    >  2:635675/1‑150 (MQ=255)

                                             ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                               <  1:613587/151‑1 (MQ=255)

                                             ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                               <  2:228114/151‑1 (MQ=255)

                                             ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                               <  2:228109/151‑1 (MQ=255)

                                              taccactaacgcccccAAACCGCCCGCGGTGAACCACGCAGATTTGGGCAGATGTGAAGAGCCGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGCTATAGAACATGAAAATGTCGCCAACAACCAAAt                                               <  2:1423974/135‑1 (MQ=255)

                                               aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                             <  1:212193/151‑1 (MQ=255)

                                               aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                             <  1:9662/151‑1 (MQ=255)

                                                   aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCTGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                         <  1:1511445/151‑1 (MQ=255)

                                                   aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                         <  2:995542/151‑1 (MQ=255)

                                                   aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                         <  1:1511444/151‑1 (MQ=255)

                                                   aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                         <  1:1511452/151‑1 (MQ=255)

                                                   aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGAATTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                         <  2:640641/151‑1 (MQ=255)

                                                            tcacacctcgcgcgGTGAAAATAGCAGATTTGGGCAGATTTGTTGATACGGAGCAA‑AG‑GCCTGCC‑G‑CAGGATATCTCTTCGGCGAGATTTTCTACGATTCTGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                 <  2:1524931/142‑1 (MQ=255)

                                                            gCAAACCGCGCGCGGTGAAAAAAGCTGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                 <  2:296940/150‑1 (MQ=255)

                                                            gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                 <  1:313607/150‑1 (MQ=255)

                                                            gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                 <  2:498522/150‑1 (MQ=255)

                                                            gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                 <  1:635167/150‑1 (MQ=255)

                                                            gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                 <  1:635173/150‑1 (MQ=255)

                                                              aaaCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                              <  2:243797/151‑1 (MQ=255)

                                                              aaaCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                              <  1:464459/151‑1 (MQ=255)

                                                                 ccGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATAGAAGGCGTAATCGTAtata                           >  2:598567/1‑148 (MQ=255)

                                                                    cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                         <  1:230479/150‑1 (MQ=255)

                                                                    cgcgagGTGATAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                         <  2:1214505/150‑1 (MQ=255)

                                                                        cgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAAt                    <  1:622801/151‑1 (MQ=255)

                                                                        cgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAAt                    >  2:632302/1‑151 (MQ=255)

                                                                           tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGTCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                  <  2:716549/150‑1 (MQ=255)

                                                                           tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                  <  1:1340912/150‑1 (MQ=255)

                                                                            gaaaattgCAGATTTGGGCAGATTTGAAGATACGGAGCTCCGG‑‑CCTGCCTC‑C‑GGCTATCTCTTCGTCGAGATTTTCTACGATTCTGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATaacg                >  2:768679/8‑148 (MQ=255)

                                                                            gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                >  2:93344/1‑151 (MQ=255)

                                                                            gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                >  1:1054048/1‑151 (MQ=255)

                                                                            gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGAAATCGAATTTACCAAAATACCg                >  2:440916/1‑151 (MQ=255)

                                                                              aaaaaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCgag              >  1:879356/1‑151 (MQ=255)

                                                                               aaacgCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑GAC‑GGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCgaga             <  1:1287849/147‑1 (MQ=255)

                                                                               aaaaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCgaga             <  2:774539/151‑1 (MQ=255)

                                                                                 aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa           >  1:720221/1‑151 (MQ=255)

                                                                                      gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa      >  2:922380/1‑151 (MQ=255)

                                                                                      gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa      >  1:1096734/1‑151 (MQ=255)

                                                                                      gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa      >  2:799810/1‑151 (MQ=255)

                                                                                      gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa      >  2:400153/1‑151 (MQ=255)

                                                                                      gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa      >  1:1455799/1‑151 (MQ=255)

                                                                                      gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAATGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa      >  2:198009/1‑151 (MQ=255)

                                                                                       aTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTc        <  1:801342/148‑1 (MQ=255)

                                                                                          tGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa  <  2:780606/151‑1 (MQ=255)

                                                                                           gggCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCC‑G‑CCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa  >  2:1294767/1‑150 (MQ=255)

||

AACTGGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCC‑G‑C‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAA  >  Exported/522338‑522579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`