breseq  version 0.37.1  revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,788,2281.C75.0% 111.2 / 42.0 44intergenic (+767/‑103)BCHEFKBB_01823/BCHEFKBB_01824hypothetical protein [NGO_1810]/pseudouridine synthase [NGO_1811]
*Exported2,073,0471.G73.3% 74.6 / 28.5 30intergenic (‑108/‑396)BCHEFKBB_02124/BCHEFKBB_02125FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
*Exported2,073,0472.G73.3% 76.1 / 28.5 30intergenic (‑108/‑396)BCHEFKBB_02124/BCHEFKBB_02125FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
*Exported1,729,7181.G72.0% 89.3 / 52.7 50coding (745/753 nt)BCHEFKBB_01772glycosyl transferase family 1 [NGO_1765]
*Exported2,073,0473.G56.7% 46.3 / 52.5 30intergenic (‑108/‑396)BCHEFKBB_02124/BCHEFKBB_02125FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
*Exported2,135,7304.G45.8% 23.5 / 102.9 48intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
*Exported1,831,9900GA38.0% 191.4 / 291.6 239intergenic (‑123/+230)BCHEFKBB_01881/BCHEFKBB_01882Opacity protein opA54 [NGO_09965]/transposase [NGO_09970]
*Exported564,7180TG30.1% 242.1 / 32.4 136N308H (AAC→CAC) uvrCexcinuclease ABC subunit C [NGO_0578]
*Exported1,887,9340TC29.7% 516.9 / 263.6 288intergenic (‑317/+264)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported1,887,9430AG27.9% 558.5 / 244.5 304intergenic (‑326/+255)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported1,887,9410CA27.0% 563.5 / 218.5 305intergenic (‑324/+257)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported1,077,9930GT26.2% 309.4 / 25.5 189E59* (GAA→TAA) BCHEFKBB_01113hypothetical protein [NGO_1132]
*Exported801,4600CA25.8% 308.5 / 33.5 163R178R (CGC→CGAcysISulfite reductase [NADPH] hemoproteinbeta‑component
*Exported1,572,2120CA24.2% 271.5 / 18.3 128M223I (ATG→ATTBCHEFKBB_01601hypothetical protein [NGO_1599]
*Exported1,383,8700GT23.7% 370.0 / 31.4 169R262L (CGT→CTT) BCHEFKBB_01424thiamine biosynthesis protein ApbE [NGO_1420]
*Exported1,891,0250AT23.1% 296.6 / 14.4 135L75Q (CTG→CAG) BCHEFKBB_01932pilus assembly protein [NGO_10270]
*Exported1,077,9870CA23.1% 358.8 / 21.9 186Q57K (CAA→AAA) BCHEFKBB_01113hypothetical protein [NGO_1132]
*Exported966,5970AT22.6% 350.5 / 10.9 164M284L (ATG→TTG) secApreprotein translocase subunit SecA [NGO_0996]
*Exported809,7770AC22.5% 328.6 / 13.2 169V142V (GTT→GTGBCHEFKBB_00821RNA methyltransferase [NGO_0819]
*Exported282,0980CA22.1% 437.5 / 21.4 205E109* (GAA→TAA) panSPantothenate precursors transporter PanS

Marginal new junction evidence (lowest skew 10 of 66 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported = 75346145 (0.810)30 (0.470)
+25 bp
5/104 4.3 53.7% intergenic (‑87/‑1007) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported = 1482412 0 (0.000)intergenic (+357/‑118) BCHEFKBB_01513/BCHEFKBB_01514 hypothetical protein [NGO_07995]/Opacity protein opA54 [NGO_1513]
* ? Exported 75303 =NA (NA)0 (0.000)
+25 bp
5/104 4.3 NA intergenic (‑44/‑1050) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported = 2038034 NA (NA)intergenic (‑43/+432) BCHEFKBB_02086/pilE Opacity protein opA54 [NGO_11100]/Fimbrial protein
* ? Exported = 213572026 (0.160)46 (0.260)
+GGGG
21/264 4.7 63.7% intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
?Exported 2135721 = 26 (0.140)intergenic (+314/‑78) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported 2132863 =122 (0.680)32 (0.220)
+TCCCCCCCCCCCCCC
18/242 4.9 32.0% intergenic (+329/‑67) BCHEFKBB_02180/BCHEFKBB_02181 glycyl‑tRNA synthetase subunit beta [NGO_2154]/glycosyl transferase [NGO_11610]
?Exported = 2135720 39 (0.230)intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported = 20730388 (0.050)47 (0.270)
+GGG
20/268 5.1 85.3% intergenic (‑99/‑405) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
?Exported 2073039 = 8 (0.040)intergenic (‑100/‑404) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
* ? Exported = 75346NA (NA)50 (0.780)
+25 bp
3/104 5.6 NA intergenic (‑87/‑1007) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported = 924691 NA (NA)intergenic (‑442/‑118) BCHEFKBB_00942/BCHEFKBB_00943 proline iminopeptidase [NGO_0949]/Opacity protein opA54 [NGO_08230]
* ? Exported = 5192886 (0.480)39 (0.240) 18/268 5.7 23.1% intergenic (‑429/+96) BCHEFKBB_00048/BCHEFKBB_00049 carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055]
?Exported 1887832 = 181 (1.100)intergenic (‑215/+366) BCHEFKBB_01931/BCHEFKBB_01932 GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
* ? Exported = 213572033 (0.200)33 (0.190)
+GGG
15/266 6.7 49.6% intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
?Exported 2135721 = 33 (0.180)intergenic (+314/‑78) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported = 695311 (0.010)54 (0.310)
+GAAGA
3/124 6.8 58.9% intergenic (‑43/‑515) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]
?Exported = 924765 76 (0.420)intergenic (‑516/‑44) BCHEFKBB_00942/BCHEFKBB_00943 proline iminopeptidase [NGO_0949]/Opacity protein opA54 [NGO_08230]
* ? Exported 69537 =118 (0.660)5 (0.060) 3/134 7.3 8.7% intergenic (‑49/‑509) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]
?Exported = 69531 51 (0.620)intergenic (‑43/‑515) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]