Predicted mutation
evidence seq id position mutation annotation gene description
RA Exported 522,455 A→G Q845R (CAG→CGG) 
T130T (ACT→ACC) 		 BCHEFKBB_00548 →
BCHEFKBB_00549 ←		 restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported522,4550AG99.0% 393.4 / ‑4.8 101Q845R (CAG→CGG) 
T130T (ACT→ACC) 		BCHEFKBB_00548
BCHEFKBB_00549		restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (44/55);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAA  >  Exported/522342‑522581
                                                                                                                 |                                                                                                                               
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:100493/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:1053860/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:1053863/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:1053929/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:685476/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:115771/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  2:345931/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:434906/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                           >  1:434910/1‑150 (MQ=255)
ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGAGTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTcaa                                                                                           >  2:636445/1‑148 (MQ=255)
 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                          <  2:1342376/150‑1 (MQ=255)
 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                          <  2:546155/150‑1 (MQ=255)
 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                          <  2:546151/150‑1 (MQ=255)
 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                          <  2:445785/150‑1 (MQ=255)
 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                          <  2:926056/150‑1 (MQ=255)
         gCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGa                                                                                  <  1:1115938/150‑1 (MQ=255)
          cTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGata                                                                                >  1:615461/1‑151 (MQ=255)
              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAACGCCGGCAAACCGCGCGCGGTGAAAACAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                             <  2:783016/150‑1 (MQ=255)
              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                             <  2:783021/150‑1 (MQ=255)
              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                            <  1:84071/151‑1 (MQ=255)
              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                            <  1:84072/151‑1 (MQ=255)
              tCGGCAGCAAAATCCATCCGACAAAATTTACCAATAAAGACGGCAAACCGCGCGCGTTGAACAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                            <  2:624535/151‑1 (MQ=255)
               ctgcagcaAAATACATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCTGTGTATAAAGCAGATTTGTGCAGATTTGAAGATACGGACCAC‑AG‑GCCTGGCGCCGGATATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           <  1:1135010/149‑1 (MQ=255)
               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGCCGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           <  2:372379/151‑1 (MQ=255)
               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           <  2:147056/151‑1 (MQ=255)
               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTAGGGCAGATTTGAAGATACGGAGCACCGG‑‑CATGCCGCCGGCTATCACTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           <  2:1272454/151‑1 (MQ=255)
                  cagcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACAt                                                                        >  1:640081/1‑151 (MQ=255)
                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           <  1:574805/146‑1 (MQ=255)
                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           >  2:574626/1‑146 (MQ=255)
                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           >  2:574634/1‑146 (MQ=255)
                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                           <  1:574813/146‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  1:594272/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  2:1413855/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  2:407289/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  2:1413857/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  2:252450/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  2:1430110/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  2:1430111/151‑1 (MQ=255)
                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                     <  1:1691233/151‑1 (MQ=255)
                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                   >  2:492496/1‑151 (MQ=255)
                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                   >  1:773552/1‑151 (MQ=255)
                        aaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAAt                                                                  >  2:676566/1‑151 (MQ=255)
                         atccatccGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATg                                                                 <  1:1692922/151‑1 (MQ=255)
                            catccGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCg                                                              >  2:1186231/1‑151 (MQ=255)
                            catccGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCg                                                              >  2:1186237/1‑151 (MQ=255)
                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                          >  2:162422/1‑151 (MQ=255)
                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                          >  1:594749/1‑151 (MQ=255)
                                 gACCAAATTTACCAAAAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                          >  2:813057/1‑150 (MQ=255)
                                  aCCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaac                                                         <  2:1067772/150‑1 (MQ=255)
                                  aCCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaac                                                         <  2:1067798/150‑1 (MQ=255)
                                  aCCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaac                                                         <  1:1288368/150‑1 (MQ=255)
                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                      >  2:727038/1‑150 (MQ=255)
                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                      >  2:1125978/1‑150 (MQ=255)
                                      aaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCa                                                    >  2:939926/1‑151 (MQ=255)
                                       aTTTACCAATAAATACGGCAAACCGCGCGCGGTTAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGAACC‑‑TGCCTGCCGCCGGCTAACTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACAAGAAAATGTCGAAAACAACCaa                                                   >  2:1183302/1‑151 (MQ=255)
                                       aTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCaa                                                   >  2:1087548/1‑151 (MQ=255)
                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                 <  2:1605719/151‑1 (MQ=255)
                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                 <  2:1605705/151‑1 (MQ=255)
                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                 <  2:1444577/151‑1 (MQ=255)
                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                               <  1:65881/151‑1 (MQ=255)
                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                              >  1:675784/1‑151 (MQ=255)
                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                         >  1:343503/1‑151 (MQ=255)
                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                         >  1:856792/1‑151 (MQ=255)
                                                   aGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGAGATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTa                                       >  1:1048747/1‑151 (MQ=255)
                                                    gACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTaa                                      >  1:331319/1‑151 (MQ=255)
                                                      cGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACCACCAAATAGAAGGCGTAAt                                     >  2:748039/1‑150 (MQ=255)
                                                      cGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAAt                                     >  2:689537/1‑150 (MQ=255)
                                                      cGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATc                                    <  1:492655/151‑1 (MQ=255)
                                                       ggCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                   >  1:108126/1‑151 (MQ=255)
                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                   <  2:906232/150‑1 (MQ=255)
                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                   <  2:108085/150‑1 (MQ=255)
                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                   <  1:59823/150‑1 (MQ=255)
                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                   <  1:59817/150‑1 (MQ=255)
                                                                cgcgcgGTGACAAAAGCAGAGTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACCTGAAAATGTCGCCAACAACCAACTTGAAGGCGTAATCGTATTTAcc                           <  2:1436640/150‑1 (MQ=255)
                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                           <  1:1512183/150‑1 (MQ=255)
                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                           <  1:510707/150‑1 (MQ=255)
                                                                    cgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCTGATATAGAAAATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATATACCAAAAt                      >  1:760687/1‑151 (MQ=255)
                                                                      gTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATa                     >  2:1375209/1‑150 (MQ=255)
                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                    <  1:26736/150‑1 (MQ=255)
                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                    <  1:26732/150‑1 (MQ=255)
                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                    <  2:1602669/150‑1 (MQ=255)
                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATAGAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                    <  2:873487/150‑1 (MQ=255)
                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                >  1:1598256/1‑137 (MQ=255)
                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                <  2:1597700/137‑1 (MQ=255)
                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                  >  2:1642913/1‑151 (MQ=255)
                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                  >  2:1642910/1‑151 (MQ=255)
                                                                           aaaaGCAGCTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACAAAATTGAAGGCGTAAACGTATTTACCAAAATACCgaga               <  2:1257760/151‑1 (MQ=255)
                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa             >  2:54896/1‑151 (MQ=255)
                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa             >  2:54975/1‑151 (MQ=255)
                                                                                cAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc            <  1:367273/149‑1 (MQ=255)
                                                                                cAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc            <  1:367256/149‑1 (MQ=255)
                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAAcc                                                     <  1:1487914/106‑1 (MQ=255)
                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAAcc                                                     >  2:1487399/1‑106 (MQ=255)
                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa        >  1:467158/1‑151 (MQ=255)
                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa        >  1:866417/1‑151 (MQ=255)
                                                                                   aTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTc          <  2:1527655/148‑1 (MQ=255)
                                                                                      tGGGCAGATTTGAAGATACGGCGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa    <  1:598262/151‑1 (MQ=255)
                                                                                      tGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCACTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa    <  2:1005265/151‑1 (MQ=255)
                                                                                         gCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaaaa  <  1:310601/150‑1 (MQ=255)
                                                                                         gCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaaaa  <  2:149480/150‑1 (MQ=255)
                                                                                                                 |                                                                                                                               
GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAA  >  Exported/522342‑522581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑