BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Exported	522,467	2 bp→CGG	coding (2546‑2547/2592 nt) coding (377‑378/462 nt)	BCHEFKBB_00548 → BCHEFKBB_00549 ←	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	522,466	1	.	C	100.0%	386.9 / NA	101	L849? (CTA→NGG) A127? (GCG→NCG)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (45/56); total (45/56)
*	Exported	522,467	0	T	G	100.0%	390.7 / NA	101	L849? (CTA→NGG) V126G (GTA→GGC)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (45/56); total (45/56)
*	Exported	522,468	0	A	G	100.0%	379.5 / NA	101	L849? (CTA→NGG) V126G (GTA→GGC)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (45/56); total (45/56)

Only 100 of 146 total aligned reads displayed.

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACTT  >  Exported/522342‑522618

|||

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:100493/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:345931/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:685476/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:115771/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:434910/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:1053929/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:1053860/1‑150 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                                                               <  2:1342376/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                                                               <  2:546151/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                                                               <  2:445785/150‑1 (MQ=255)

         gCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGa                                                                                                                       <  1:1115938/150‑1 (MQ=255)

          cTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGata                                                                                                                     >  1:615461/1‑151 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAACGCCGGCAAACCGCGCGCGGTGAAAACAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                                                                  <  2:783016/150‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                                                                  <  2:783021/150‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                                                                 <  1:84072/151‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                                                                 <  1:84071/151‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACAAAATTTACCAATAAAGACGGCAAACCGCGCGCGTTGAACAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                                                                 <  2:624535/151‑1 (MQ=255)

               ctgcagcaAAATACATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCTGTGTATAAAGCAGATTTGTGCAGATTTGAAGATACGGACCAC‑AG‑GCCTGGCGCCGGATATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  1:1135010/149‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  2:147056/151‑1 (MQ=255)

                  cagcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACAt                                                                                                             >  1:640081/1‑151 (MQ=255)

                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  1:574805/146‑1 (MQ=255)

                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                >  2:574634/1‑146 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  2:1430111/151‑1 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  1:594272/151‑1 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  2:1413855/151‑1 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  2:1430110/151‑1 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  2:407289/151‑1 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  1:1691233/151‑1 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                                                        >  2:492496/1‑151 (MQ=255)

                        aaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAAt                                                                                                       >  2:676566/1‑151 (MQ=255)

                         atccatccGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATg                                                                                                      <  1:1692922/151‑1 (MQ=255)

                            catccGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCg                                                                                                   >  2:1186231/1‑151 (MQ=255)

                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                                                               >  2:162422/1‑151 (MQ=255)

                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                                                               >  1:594749/1‑151 (MQ=255)

                                  aCCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaac                                                                                              <  1:1288368/150‑1 (MQ=255)

                                  aCCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaac                                                                                              <  2:1067798/150‑1 (MQ=255)

                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                                                           >  2:727038/1‑150 (MQ=255)

                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                                                           >  2:1125978/1‑150 (MQ=255)

                                      aaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCa                                                                                         >  2:939926/1‑151 (MQ=255)

                                       aTTTACCAATAAATACGGCAAACCGCGCGCGGTTAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGAACC‑‑TGCCTGCCGCCGGCTAACTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACAAGAAAATGTCGAAAACAACCaa                                                                                        >  2:1183302/1‑151 (MQ=255)

                                       aTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCaa                                                                                        >  2:1087548/1‑151 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                                                      <  2:1444577/151‑1 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                                                      <  2:1605719/151‑1 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                                                                   >  1:675784/1‑151 (MQ=255)

                                                   aGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGAGATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTa                                                                            >  1:1048747/1‑151 (MQ=255)

                                                    gACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTaa                                                                           >  1:331319/1‑151 (MQ=255)

                                                       ggCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        >  1:108126/1‑151 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        <  2:108085/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        <  2:906232/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        <  1:59823/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                                                                <  1:510707/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                                                                <  1:1512183/150‑1 (MQ=255)

                                                                    cgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCTGATATAGAAAATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATATACCAAAAt                                                           >  1:760687/1‑151 (MQ=255)

                                                                      gTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATa                                                          >  2:1375209/1‑150 (MQ=255)

                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                                                         <  2:1602669/150‑1 (MQ=255)

                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                                                         <  1:26732/150‑1 (MQ=255)

                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATAGAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                                                         <  2:873487/150‑1 (MQ=255)

                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                                                     >  1:1598256/1‑137 (MQ=255)

                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                                                       >  2:1642913/1‑151 (MQ=255)

                                                                           aaaaGCAGCTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACAAAATTGAAGGCGTAAACGTATTTACCAAAATACCgaga                                                    <  2:1257760/151‑1 (MQ=255)

                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa                                                  >  2:54975/1‑151 (MQ=255)

                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa                                                  >  2:54896/1‑151 (MQ=255)

                                                                                cAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc                                                 <  1:367256/149‑1 (MQ=255)

                                                                                cAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc                                                 <  1:367273/149‑1 (MQ=255)

                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa                                             >  1:866417/1‑151 (MQ=255)

                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa                                             >  1:467158/1‑151 (MQ=255)

                                                                                   aTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTc                                               <  2:1527655/148‑1 (MQ=255)

                                                                                      tGGGCAGATTTGAAGATACGGCGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa                                         <  1:598262/151‑1 (MQ=255)

                                                                                      tGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCACTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa                                         <  2:1005265/151‑1 (MQ=255)

                                                                                         gCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaaaa                                       <  2:149480/150‑1 (MQ=255)

                                                                                         gCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaaaa                                       <  1:310601/150‑1 (MQ=255)

                                                                                                                   ggcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGCTTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTCTTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCGCGTa              >  1:1433220/14‑151 (MQ=255)

                                                                                                                   ggcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTa              >  2:1616712/14‑151 (MQ=255)

                                                                                                                   ggcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTa              >  2:1616721/14‑151 (MQ=255)

                                                                                                                   ggcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTa              >  2:245529/14‑151 (MQ=255)

                                                                                                                    gcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAt             >  2:1085562/13‑151 (MQ=255)

                                                                                                                    gcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAt             >  2:454471/13‑151 (MQ=255)

                                                                                                                    gcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAt             >  2:454470/13‑151 (MQ=255)

                                                                                                                     cctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAtt            <  2:1338088/140‑1 (MQ=255)

                                                                                                                      ctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTc           <  2:1447130/141‑1 (MQ=255)

                                                                                                                        gccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCg          >  1:757363/9‑150 (MQ=255)

                                                                                                                        gccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCg          >  2:726383/9‑150 (MQ=255)

                                                                                                                        gccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGAGATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCg          >  2:300930/9‑150 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGTCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGc         >  1:1058450/8‑150 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAACTCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  1:987517/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:596633/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:639885/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  1:1186640/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:969774/144‑1 (MQ=255)

                                                                                                                          cgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCcggc                         <  2:1227769/127‑1 (MQ=255)

                                                                                                                          cgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGccc       <  1:740988/145‑1 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  2:283446/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  2:283442/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  2:283406/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  2:1604335/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  1:914293/3‑149 (MQ=255)

                                                                                                                               gctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACtt  >  1:122676/2‑151 (MQ=255)

                                                                                                                               gctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACtt  >  1:437144/2‑151 (MQ=255)

                                                                                                                               gctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACt   <  1:174279/149‑1 (MQ=255)

                                                                                                                                ctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATccc                                   >  1:1365276/1‑117 (MQ=255)

|||

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACTT  >  Exported/522342‑522618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`