breseq  version 0.37.1  revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 42,301 5 bp→5 bp coding (156‑160/462 nt) BCHEFKBB_00042 ← acetyl‑CoA carboxylase [NGO_0045]
RA 522,455 A→G Q845R (CAG→CGG) 
T130T (ACT→ACC
BCHEFKBB_00548 →
BCHEFKBB_00549 ←
restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
RA 522,457 4 bp→CC coding (2536‑2539/2592 nt)
coding (385‑388/462 nt)
BCHEFKBB_00548 →
BCHEFKBB_00549 ←
restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
RA 522,467 2 bp→CGG coding (2546‑2547/2592 nt)
coding (377‑378/462 nt)
BCHEFKBB_00548 →
BCHEFKBB_00549 ←
restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
RA 1,162,857 C→T G411D (GGC→GAC)  BCHEFKBB_01205 ← long‑chain fatty acid‑‑CoA ligase [NGO_1213]
RA 2,073,047 (G)9→12 intergenic (‑108/‑396) BCHEFKBB_02124 ← / → BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
RA 2,134,653 (G)9→13 coding (166/183 nt) BCHEFKBB_02183 → hypothetical protein
RA 2,135,730 (G)10→13 intergenic (+323/‑69) BCHEFKBB_02184 → / → BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ Exported 745777–745878 747126–745880 3–1350 118 [116] [114] 120 BCHEFKBB_00758–[BCHEFKBB_00760] BCHEFKBB_00758,BCHEFKBB_00759,[BCHEFKBB_00760]
* * ÷ Exported 1072583–1075092 1077577–1075129 38–4995 119 [117] [117] 119 [BCHEFKBB_01101]–BCHEFKBB_01111 [BCHEFKBB_01101],BCHEFKBB_01102,BCHEFKBB_01103,BCHEFKBB_01104,BCHEFKBB_01105,BCHEFKBB_01106,BCHEFKBB_01107,BCHEFKBB_01108,BCHEFKBB_01109,BCHEFKBB_01110,BCHEFKBB_01111
* * ÷ Exported 1159995–1160223 1160538–1160229 7–544 118 [116] [112] 119 [BCHEFKBB_01202]–BCHEFKBB_01203 [BCHEFKBB_01202],BCHEFKBB_01203
* * ÷ Exported 1215585–1220269 1223583 3315–7999 119 [117] [117] 118 BCHEFKBB_01257–BCHEFKBB_01268 BCHEFKBB_01257,BCHEFKBB_01258,BCHEFKBB_01259,BCHEFKBB_01260,BCHEFKBB_01261,BCHEFKBB_01262,BCHEFKBB_01263,BCHEFKBB_01264,BCHEFKBB_01265,BCHEFKBB_01266,BCHEFKBB_01267,BCHEFKBB_01268
* * ÷ Exported 2065325–2065503 2065934–2065619 117–610 131 [114] [117] 127 [BCHEFKBB_02117]–[BCHEFKBB_02118] [BCHEFKBB_02117],[BCHEFKBB_02118]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported = 695311 (0.010)81 (0.710)
+65 bp
5/4 0.0 62.6% intergenic (‑43/‑515) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]
?Exported = 1034998 172 (0.840)intergenic (+414/‑44) BCHEFKBB_01058/BCHEFKBB_01059 hypothetical protein/Opacity protein opA54 [NGO_05620]
* ? Exported = 75346203 (0.990)144 (0.950) 26/214 2.7 49.2% intergenic (‑87/‑1007) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported = 1482447 NA (NA)intergenic (+392/‑83) BCHEFKBB_01513/BCHEFKBB_01514 hypothetical protein [NGO_07995]/Opacity protein opA54 [NGO_1513]