breseq  version 0.37.1  revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 41 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,788,2281.C79.1% 117.1 / 35.3 43intergenic (+767/‑103)BCHEFKBB_01823/BCHEFKBB_01824hypothetical protein [NGO_1810]/pseudouridine synthase [NGO_1811]
*Exported1,729,7181.G76.7% 201.5 / 77.5 86coding (745/753 nt)BCHEFKBB_01772glycosyl transferase family 1 [NGO_1765]
*Exported2,134,6535.G74.2% 61.1 / 29.2 32coding (166/183 nt)BCHEFKBB_02183hypothetical protein
*Exported2,073,0474.G69.2% 45.8 / 29.6 26intergenic (‑108/‑396)BCHEFKBB_02124/BCHEFKBB_02125FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
*Exported2,135,7304.G59.2% ‑1.3 / 86.3 49intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
*Exported1,831,9900GA42.4% 163.8 / inf 301intergenic (‑123/+230)BCHEFKBB_01881/BCHEFKBB_01882Opacity protein opA54 [NGO_09965]/transposase [NGO_09970]
*Exported1,396,2110CG31.0% 172.1 / 27.9 87A93G (GCG→GGG) BCHEFKBB_01437ABC transporter permease [NGO_1433]
*Exported260,1260TG29.0% 445.1 / 160.2 202intergenic (+226/+249)BCHEFKBB_00257/BCHEFKBB_00258ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261]
*Exported260,1090CG28.8% 473.0 / 185.8 200intergenic (+209/+266)BCHEFKBB_00257/BCHEFKBB_00258ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261]
*Exported260,1450GA27.0% 431.0 / 150.7 199intergenic (+245/+230)BCHEFKBB_00257/BCHEFKBB_00258ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261]
*Exported1,690,8550TG26.5% 261.3 / 14.4 134A57A (GCT→GCGBCHEFKBB_01736peptidase [NGO_1729]
*Exported553,2010TA26.2% 237.4 / 15.9 130M33L (ATG→TTG) BCHEFKBB_00575creA protein [NGO_0570]
*Exported999,0790GT25.8% 360.3 / 26.2 178Y7* (TAC→TAABCHEFKBB_01021hypothetical protein
*Exported1,887,9340TC25.8% 602.8 / 216.6 285intergenic (‑317/+264)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported1,887,9410CA25.6% 644.2 / 195.4 297intergenic (‑324/+257)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported275,0310CA24.7% 315.1 / 14.4 168E611* (GAA→TAA) BCHEFKBB_00272peptidase [NGO_0275]
*Exported58,9360TG24.6% 401.7 / 35.7 192intergenic (+74/‑267)BCHEFKBB_00053/BCHEFKBB_00054thioredoxin [NGO_0057]/homoprotocatechuate degradative operon repressor [NGO_0058]
*Exported1,887,9430AG24.4% 639.6 / 211.0 297intergenic (‑326/+255)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported260,0600AG24.1% 561.8 / 167.8 237intergenic (+160/+315)BCHEFKBB_00257/BCHEFKBB_00258ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261]
*Exported1,671,3840CA24.1% 270.8 / 16.8 116Q18K (CAA→AAA) BCHEFKBB_01719ribonuclease II [NGO_1713]

Marginal new junction evidence (lowest skew 10 of 70 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported = 21346442 (0.010)74 (0.370)
+GGGGG
24/264 4.8 97.4% coding (157/183 nt) BCHEFKBB_02183 hypothetical protein
?Exported 2134645 = 2 (0.010)coding (158/183 nt) BCHEFKBB_02183 hypothetical protein
* ? Exported 2132863 =140 (0.680)38 (0.220)
+TCCCCCCCCCCCCCC
20/242 5.2 31.8% intergenic (+329/‑67) BCHEFKBB_02180/BCHEFKBB_02181 glycyl‑tRNA synthetase subunit beta [NGO_2154]/glycosyl transferase [NGO_11610]
?Exported = 2135720 53 (0.280)intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported = 213572022 (0.110)71 (0.350)
+GGGG
22/264 5.4 76.2% intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
?Exported 2135721 = 22 (0.110)intergenic (+314/‑78) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported 51843 =116 (0.560)82 (0.420)
+ACTTTCGT
22/276 5.8 33.5% intergenic (‑344/+181) BCHEFKBB_00048/BCHEFKBB_00049 carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055]
?Exported 2092176 = 228 (1.110)intergenic (+321/+572) BCHEFKBB_02138/BCHEFKBB_02139 tRNA modification GTPase TrmE [NGO_2107]/ligand‑gated channel protein [NGO_2109]
* ? Exported = 260158191 (0.930)43 (0.230)
+ATTCCCGCCTGCGC
20/264 6.0 19.0% intergenic (+258/+217) BCHEFKBB_00257/BCHEFKBB_00258 ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261]
?Exported = 1888008 215 (1.040)intergenic (‑391/+190) BCHEFKBB_01931/BCHEFKBB_01932 GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
* ? Exported = 20730387 (0.040)47 (0.230)
+GGGG
20/266 6.1 87.0% intergenic (‑99/‑405) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
?Exported 2073039 = 7 (0.030)intergenic (‑100/‑404) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
* ? Exported = 695311 (0.010)81 (0.410)
+GAAGA
5/124 6.2 97.5% intergenic (‑43/‑515) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]
?Exported 2038035 = NA (NA)intergenic (‑44/+431) BCHEFKBB_02086/pilE Opacity protein opA54 [NGO_11100]/Fimbrial protein
* ? Exported 69537 =60 (0.290)7 (0.070) 5/134 6.8 14.2% intergenic (‑49/‑509) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]
?Exported = 69531 57 (0.600)intergenic (‑43/‑515) BCHEFKBB_00062/BCHEFKBB_00063 Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068]
* ? Exported = 2043450190 (0.920)16 (0.110) 12/216 7.2 18.6% coding (262/495 nt) BCHEFKBB_02092 hypothetical protein
?Exported 2046839 = 0 (0.000)coding (333/390 nt) BCHEFKBB_02098 hypothetical protein [NGO_11165]
* ? Exported = 2043450190 (0.920)16 (0.110) 12/216 7.2 18.6% coding (262/495 nt) BCHEFKBB_02092 hypothetical protein
?Exported 2043779 = 0 (0.000)coding (252/309 nt) BCHEFKBB_02093 hypothetical protein [NGO_11165]