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breseq version 0.37.1 revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 41 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,788,228 | 1 | . | C | 79.1% | 117.1 / 35.3 | 43 | intergenic (+767/‑103) | BCHEFKBB_01823/BCHEFKBB_01824 | hypothetical protein [NGO_1810]/pseudouridine synthase [NGO_1811] |
| * | Exported | 1,729,718 | 1 | . | G | 76.7% | 201.5 / 77.5 | 86 | coding (745/753 nt) | BCHEFKBB_01772 | glycosyl transferase family 1 [NGO_1765] |
| * | Exported | 2,134,653 | 5 | . | G | 74.2% | 61.1 / 29.2 | 32 | coding (166/183 nt) | BCHEFKBB_02183 | hypothetical protein |
| * | Exported | 2,073,047 | 4 | . | G | 69.2% | 45.8 / 29.6 | 26 | intergenic (‑108/‑396) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] |
| * | Exported | 2,135,730 | 4 | . | G | 59.2% | ‑1.3 / 86.3 | 49 | intergenic (+323/‑69) | BCHEFKBB_02184/BCHEFKBB_02185 | glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158] |
| * | Exported | 1,831,990 | 0 | G | A | 42.4% | 163.8 / inf | 301 | intergenic (‑123/+230) | BCHEFKBB_01881/BCHEFKBB_01882 | Opacity protein opA54 [NGO_09965]/transposase [NGO_09970] |
| * | Exported | 1,396,211 | 0 | C | G | 31.0% | 172.1 / 27.9 | 87 | A93G (GCG→GGG) | BCHEFKBB_01437 | ABC transporter permease [NGO_1433] |
| * | Exported | 260,126 | 0 | T | G | 29.0% | 445.1 / 160.2 | 202 | intergenic (+226/+249) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 260,109 | 0 | C | G | 28.8% | 473.0 / 185.8 | 200 | intergenic (+209/+266) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 260,145 | 0 | G | A | 27.0% | 431.0 / 150.7 | 199 | intergenic (+245/+230) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 1,690,855 | 0 | T | G | 26.5% | 261.3 / 14.4 | 134 | A57A (GCT→GCG) | BCHEFKBB_01736 | peptidase [NGO_1729] |
| * | Exported | 553,201 | 0 | T | A | 26.2% | 237.4 / 15.9 | 130 | M33L (ATG→TTG) | BCHEFKBB_00575 | creA protein [NGO_0570] |
| * | Exported | 999,079 | 0 | G | T | 25.8% | 360.3 / 26.2 | 178 | Y7* (TAC→TAA) | BCHEFKBB_01021 | hypothetical protein |
| * | Exported | 1,887,934 | 0 | T | C | 25.8% | 602.8 / 216.6 | 285 | intergenic (‑317/+264) | BCHEFKBB_01931/BCHEFKBB_01932 | GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270] |
| * | Exported | 1,887,941 | 0 | C | A | 25.6% | 644.2 / 195.4 | 297 | intergenic (‑324/+257) | BCHEFKBB_01931/BCHEFKBB_01932 | GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270] |
| * | Exported | 275,031 | 0 | C | A | 24.7% | 315.1 / 14.4 | 168 | E611* (GAA→TAA) | BCHEFKBB_00272 | peptidase [NGO_0275] |
| * | Exported | 58,936 | 0 | T | G | 24.6% | 401.7 / 35.7 | 192 | intergenic (+74/‑267) | BCHEFKBB_00053/BCHEFKBB_00054 | thioredoxin [NGO_0057]/homoprotocatechuate degradative operon repressor [NGO_0058] |
| * | Exported | 1,887,943 | 0 | A | G | 24.4% | 639.6 / 211.0 | 297 | intergenic (‑326/+255) | BCHEFKBB_01931/BCHEFKBB_01932 | GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270] |
| * | Exported | 260,060 | 0 | A | G | 24.1% | 561.8 / 167.8 | 237 | intergenic (+160/+315) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 1,671,384 | 0 | C | A | 24.1% | 270.8 / 16.8 | 116 | Q18K (CAA→AAA) | BCHEFKBB_01719 | ribonuclease II [NGO_1713] |
| Marginal new junction evidence (lowest skew 10 of 70 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | Exported | = 2134644 | 2 (0.010) | 74 (0.370) +GGGGG |
24/264 | 4.8 | 97.4% | coding (157/183 nt) | BCHEFKBB_02183 | hypothetical protein |
| ? | Exported | 2134645 = | 2 (0.010) | coding (158/183 nt) | BCHEFKBB_02183 | hypothetical protein | |||||
| * | ? | Exported | 2132863 = | 140 (0.680) | 38 (0.220) +TCCCCCCCCCCCCCC |
20/242 | 5.2 | 31.8% | intergenic (+329/‑67) | BCHEFKBB_02180/BCHEFKBB_02181 | glycyl‑tRNA synthetase subunit beta [NGO_2154]/glycosyl transferase [NGO_11610] |
| ? | Exported | = 2135720 | 53 (0.280) | intergenic (+313/‑79) | BCHEFKBB_02184/BCHEFKBB_02185 | glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158] | |||||
| * | ? | Exported | = 2135720 | 22 (0.110) | 71 (0.350) +GGGG |
22/264 | 5.4 | 76.2% | intergenic (+313/‑79) | BCHEFKBB_02184/BCHEFKBB_02185 | glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158] |
| ? | Exported | 2135721 = | 22 (0.110) | intergenic (+314/‑78) | BCHEFKBB_02184/BCHEFKBB_02185 | glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158] | |||||
| * | ? | Exported | 51843 = | 116 (0.560) | 82 (0.420) +ACTTTCGT |
22/276 | 5.8 | 33.5% | intergenic (‑344/+181) | BCHEFKBB_00048/BCHEFKBB_00049 | carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055] |
| ? | Exported | 2092176 = | 228 (1.110) | intergenic (+321/+572) | BCHEFKBB_02138/BCHEFKBB_02139 | tRNA modification GTPase TrmE [NGO_2107]/ligand‑gated channel protein [NGO_2109] | |||||
| * | ? | Exported | = 260158 | 191 (0.930) | 43 (0.230) +ATTCCCGCCTGCGC |
20/264 | 6.0 | 19.0% | intergenic (+258/+217) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| ? | Exported | = 1888008 | 215 (1.040) | intergenic (‑391/+190) | BCHEFKBB_01931/BCHEFKBB_01932 | GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270] | |||||
| * | ? | Exported | = 2073038 | 7 (0.040) | 47 (0.230) +GGGG |
20/266 | 6.1 | 87.0% | intergenic (‑99/‑405) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] |
| ? | Exported | 2073039 = | 7 (0.030) | intergenic (‑100/‑404) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] | |||||
| * | ? | Exported | = 69531 | 1 (0.010) | 81 (0.410) +GAAGA |
5/124 | 6.2 | 97.5% | intergenic (‑43/‑515) | BCHEFKBB_00062/BCHEFKBB_00063 | Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068] |
| ? | Exported | 2038035 = | NA (NA) | intergenic (‑44/+431) | BCHEFKBB_02086/pilE | Opacity protein opA54 [NGO_11100]/Fimbrial protein | |||||
| * | ? | Exported | 69537 = | 60 (0.290) | 7 (0.070) | 5/134 | 6.8 | 14.2% | intergenic (‑49/‑509) | BCHEFKBB_00062/BCHEFKBB_00063 | Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068] |
| ? | Exported | = 69531 | 57 (0.600) | intergenic (‑43/‑515) | BCHEFKBB_00062/BCHEFKBB_00063 | Opacity protein opA54 [NGO_00350]/riboflavin kinase [NGO_0068] | |||||
| * | ? | Exported | = 2043450 | 190 (0.920) | 16 (0.110) | 12/216 | 7.2 | 18.6% | coding (262/495 nt) | BCHEFKBB_02092 | hypothetical protein |
| ? | Exported | 2046839 = | 0 (0.000) | coding (333/390 nt) | BCHEFKBB_02098 | hypothetical protein [NGO_11165] | |||||
| * | ? | Exported | = 2043450 | 190 (0.920) | 16 (0.110) | 12/216 | 7.2 | 18.6% | coding (262/495 nt) | BCHEFKBB_02092 | hypothetical protein |
| ? | Exported | 2043779 = | 0 (0.000) | coding (252/309 nt) | BCHEFKBB_02093 | hypothetical protein [NGO_11165] | |||||