mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.37.1 revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 49 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,729,718 | 1 | . | G | 78.4% | 199.2 / 60.6 | 74 | coding (745/753 nt) | BCHEFKBB_01772 | glycosyl transferase family 1 [NGO_1765] |
| * | Exported | 2,073,047 | 2 | . | G | 76.3% | 104.2 / 31.9 | 38 | intergenic (‑108/‑396) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] |
| * | Exported | 2,073,047 | 3 | . | G | 57.9% | 75.6 / 63.9 | 38 | intergenic (‑108/‑396) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] |
| * | Exported | 260,109 | 0 | C | G | 32.3% | 476.9 / 255.1 | 232 | intergenic (+209/+266) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 1,383,884 | 0 | G | T | 31.2% | 328.7 / 33.6 | 177 | D267Y (GAT→TAT) | BCHEFKBB_01424 | thiamine biosynthesis protein ApbE [NGO_1420] |
| * | Exported | 564,718 | 0 | T | G | 31.2% | 291.1 / 41.4 | 157 | N308H (AAC→CAC) | uvrC | excinuclease ABC subunit C [NGO_0578] |
| * | Exported | 260,126 | 0 | T | G | 30.4% | 527.3 / 221.7 | 244 | intergenic (+226/+249) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 1,831,990 | 0 | G | A | 30.0% | 393.5 / 228.8 | 251 | intergenic (‑123/+230) | BCHEFKBB_01881/BCHEFKBB_01882 | Opacity protein opA54 [NGO_09965]/transposase [NGO_09970] |
| * | Exported | 260,060 | 0 | A | G | 28.8% | 517.1 / 212.1 | 246 | intergenic (+160/+315) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 55,573 | 0 | T | A | 28.8% | 105.4 / 10.3 | 67 | H30Q (CAT→CAA) | BCHEFKBB_00050 | hypothetical protein |
| * | Exported | 712,461 | 0 | C | A | 27.4% | 151.4 / 18.5 | 87 | intergenic (‑67/+143) | BCHEFKBB_00721/BCHEFKBB_00722 | 2‑dehydro‑3‑deoxyphosphogluconate aldolase[NGO_0713]/phosphogluconate dehydratase [NGO_0714] |
| * | Exported | 1,891,267 | 0 | T | G | 27.4% | 203.9 / 13.1 | 106 | T25P (ACC→CCC) | BCHEFKBB_01933 | hypothetical protein |
| * | Exported | 260,145 | 0 | G | A | 26.8% | 563.3 / 198.4 | 251 | intergenic (+245/+230) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| * | Exported | 275,031 | 0 | C | A | 26.6% | 360.7 / 21.4 | 184 | E611* (GAA→TAA) | BCHEFKBB_00272 | peptidase [NGO_0275] |
| * | Exported | 2,026,054 | 0 | C | A | 25.4% | 365.8 / 50.0 | 173 | F68L (TTC→TTA) | BCHEFKBB_02075 | ferrous iron transporter [NGO_2050] |
| * | Exported | 948,535 | 0 | A | C | 25.4% | 392.2 / 41.0 | 205 | V511V (GTT→GTG) | BCHEFKBB_00967 | thiol:disulfide interchange protein [NGO_0978] |
| * | Exported | 1,891,025 | 0 | A | T | 25.3% | 322.8 / 17.0 | 150 | L75Q (CTG→CAG) | BCHEFKBB_01932 | pilus assembly protein [NGO_10270] |
| * | Exported | 1,914,673 | 0 | C | A | 25.3% | 350.0 / 14.5 | 158 | S183Y (TCC→TAC) | BCHEFKBB_01956 | electron transfer flavoprotein subunit beta[NGO_1936] |
| * | Exported | 1,383,863 | 0 | G | T | 25.1% | 402.8 / 32.4 | 184 | D260Y (GAT→TAT) | BCHEFKBB_01424 | thiamine biosynthesis protein ApbE [NGO_1420] |
| * | Exported | 1,383,870 | 0 | G | T | 25.0% | 361.2 / 34.0 | 180 | R262L (CGT→CTT) | BCHEFKBB_01424 | thiamine biosynthesis protein ApbE [NGO_1420] |
| Marginal new junction evidence (lowest skew 10 of 71 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | Exported | 51843 = | 113 (0.550) | 93 (0.480) +ACTTTCGT |
25/278 | 4.7 | 36.5% | intergenic (‑344/+181) | BCHEFKBB_00048/BCHEFKBB_00049 | carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055] |
| ? | Exported | 2092176 = | 229 (1.110) | intergenic (+321/+572) | BCHEFKBB_02138/BCHEFKBB_02139 | tRNA modification GTPase TrmE [NGO_2107]/ligand‑gated channel protein [NGO_2109] | |||||
| * | ? | Exported | = 260182 | 202 (0.980) | 33 (0.230) +42 bp |
15/210 | 5.4 | 18.7% | intergenic (+282/+193) | BCHEFKBB_00257/BCHEFKBB_00258 | ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261] |
| ? | Exported | = 1887026 | 202 (0.980) | intergenic (+175/+307) | BCHEFKBB_01930/BCHEFKBB_01931 | membrane protein [NGO_1910]/GIY‑YIG nuclease [NGO_1911] | |||||
| * | ? | Exported | = 1887130 | 215 (1.050) | 23 (0.160) | 14/210 | 5.8 | 13.7% | intergenic (+279/+203) | BCHEFKBB_01930/BCHEFKBB_01931 | membrane protein [NGO_1910]/GIY‑YIG nuclease [NGO_1911] |
| ? | Exported | 1887862 = | 136 (0.930) | intergenic (‑245/+336) | BCHEFKBB_01931/BCHEFKBB_01932 | GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270] | |||||
| * | ? | Exported | = 2134644 | 2 (0.010) | 54 (0.270) +GGGGG |
19/266 | 6.1 | 96.4% | coding (157/183 nt) | BCHEFKBB_02183 | hypothetical protein |
| ? | Exported | 2134645 = | 2 (0.010) | coding (158/183 nt) | BCHEFKBB_02183 | hypothetical protein | |||||
| * | ? | Exported | 1034985 = | 175 (0.850) | 12 (0.160) +40 bp |
3/106 | 6.2 | 19.7% | intergenic (+401/‑57) | BCHEFKBB_01058/BCHEFKBB_01059 | hypothetical protein/Opacity protein opA54 [NGO_05620] |
| ? | Exported | = 1231240 | 63 (0.490) | intergenic (+681/‑103) | BCHEFKBB_01273/BCHEFKBB_01274 | hypothetical protein [NGO_1277]/Opacity protein opA54 [NGO_06725] | |||||
| * | ? | Exported | 51843 = | 113 (0.550) | 73 (0.380) +ACTTTCGT |
20/278 | 6.2 | 33.4% | intergenic (‑344/+181) | BCHEFKBB_00048/BCHEFKBB_00049 | carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055] |
| ? | Exported | 2043064 = | 195 (0.950) | intergenic (‑111/+153) | BCHEFKBB_02091/BCHEFKBB_02092 | large pilS cassette [NGO_11130]/hypothetical protein | |||||
| * | ? | Exported | 51843 = | 113 (0.550) | 73 (0.380) +ACTTTCGT |
20/278 | 6.2 | 31.5% | intergenic (‑344/+181) | BCHEFKBB_00048/BCHEFKBB_00049 | carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055] |
| ? | Exported | 2016917 = | 223 (1.080) | intergenic (‑59/+219) | BCHEFKBB_02066/BCHEFKBB_02067 | hypothetical protein [NGO_10985]/hypothetical protein | |||||
| * | ? | Exported | = 51928 | 123 (0.600) | 27 (0.140) | 18/270 | 6.6 | 16.4% | intergenic (‑429/+96) | BCHEFKBB_00048/BCHEFKBB_00049 | carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055] |
| ? | Exported | 1887832 = | 162 (0.860) | intergenic (‑215/+366) | BCHEFKBB_01931/BCHEFKBB_01932 | GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270] | |||||
| * | ? | Exported | = 2073038 | 10 (0.050) | 42 (0.210) +GGG |
17/270 | 7.0 | 79.8% | intergenic (‑99/‑405) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] |
| ? | Exported | 2073039 = | 11 (0.050) | intergenic (‑100/‑404) | BCHEFKBB_02124/BCHEFKBB_02125 | FetA [NGO_2093]/molecular chaperone GroES [NGO_2094] | |||||
| * | ? | Exported | = 68368 | 128 (0.620) | 119 (0.640) +ACGGTGTTGTCGGGG |
16/264 | 7.1 | 53.9% | intergenic (+373/+431) | BCHEFKBB_00061/BCHEFKBB_00062 | tyrosyl‑tRNA synthetase [NGO_0066]/Opacity protein opA54 [NGO_00350] |
| ? | Exported | = 925607 | 99 (0.480) | intergenic (+100/‑253) | BCHEFKBB_00943/BCHEFKBB_00944 | Opacity protein opA54 [NGO_08230]/TonB‑dependent receptor [NGO_0952] | |||||