Predicted mutation
evidence seq id position mutation annotation gene description
RA Exported 2,135,730 (G)10→12 intergenic (+323/‑69) BCHEFKBB_02184 → / → BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,135,7301.G88.4% 105.0 / 14.1 43intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base G (37/1);  total (41/2)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*Exported2,135,7302.G88.4% 103.3 / 14.1 43intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base G (37/1);  total (41/2)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATGACGGCTCGACGGACGGCACGCCCGCCATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGG‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTGC  >  Exported/2135580‑2135878
                                                                                                                                                                  ||                                                                                                                                                           
cGATGACGGCTCGACGGACGGCACGCCCGCCATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑gggg‑‑‑‑gg‑gggg                                                                                                                                                               <  1:1374450/151‑1 (MQ=255)
               ggacggCAGGCCCGCCTTGGCCCGGCGTTTCCAATCACAGGCCGGCTGTATCTGTATATTTTCCACACCCCGAATTTTGGGCTTTACCGCATCTTTACACAGCGGGGGGGACGAATTGTCAACTTGGG‑‑‑‑GGGG‑‑‑‑GG‑GGGG‑‑‑‑‑A‑‑‑‑ATATATTgcgc                                                                                                                                          <  2:480540/150‑1 (MQ=255)
               ggacggCACGCCCGCCATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑gggg‑‑‑‑gg‑ggggggggaaaatattgcg                                                                                                                                                >  1:696817/1‑136 (MQ=255)
                    gCACGCCCGCCATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTgcgcgcc                                                                                                                                       >  1:930648/1‑149 (MQ=38)
                    gCACGCCCGCCATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCa                                                                                                                                       >  1:930632/1‑150 (MQ=38)
                           cgccATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGTGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATTTTGCGCGCACCGATGc                                                                                                                                >  1:1268120/1‑150 (MQ=37)
                             ccATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCcga                                                                                                                             >  2:985887/1‑151 (MQ=37)
                             ccATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCcga                                                                                                                             >  2:1213716/1‑151 (MQ=37)
                             ccATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCcga                                                                                                                             >  1:181522/1‑151 (MQ=37)
                             ccATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCCCCCCGATGCcga                                                                                                                             >  1:181523/1‑151 (MQ=37)
                             ccATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATATGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCcga                                                                                                                             >  1:1173920/1‑151 (MQ=37)
                             ccATGGCCGGGCGTTTCCACGAACCGGACGGCAGGATCTTGTTTATTCCCATTCCCCGATTTTTGGGCTTTATCGCCTCTTTACCCCTGGGGCTGGCCGAATGGGCCA‑‑CGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGG‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCcgac                                                                                                                            <  2:802084/150‑1 (MQ=255)
                                 tGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGat                                                                                                                         >  1:1294210/1‑151 (MQ=37)
                                 tGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGata                                                                                                                        >  1:327089/1‑151 (MQ=255)
                                       gCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCCCCGATGCCGACGATATTGc                                                                                                                    >  1:358847/1‑150 (MQ=25)
                                       gCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGG‑‑‑‑‑GGGGGAAAAATTGCCCGCACCCATGCCGACGATATTGc                                                                                                                    >  1:358806/1‑150 (MQ=25)
                                            tCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCAccc                                                                                                               >  2:47855/1‑150 (MQ=25)
                                            tCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATAAATTGCGCCCACCGATGCCGACGAGATTGCCtacc                                                                                                               >  2:47863/1‑147 (MQ=25)
                                             ccAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGG‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCAACCCCCgg                                                                                                           >  1:674254/1‑151 (MQ=25)
                                                 gAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCgg                                                                                                           >  1:60248/1‑149 (MQ=21)
                                                 gAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCgg                                                                                                           >  1:395476/1‑149 (MQ=21)
                                                    cAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTTCCACCCCCGGCTGGAt                                                                                                     >  1:413798/1‑151 (MQ=21)
                                                    cAGGACGGCAGGATCAGGATAAATTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCTGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTTCGCGAACCCATGCCCACCAAATTACCACCCCCggatgtat                                                                                                     >  1:413786/1‑145 (MQ=19)
                                                      ggACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCGGGAtt                                                                                                    >  2:241553/1‑151 (MQ=21)
                                                      ggACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACCATATTGCCTCCCCCGGCTGGATTg                                                                                                   >  1:1316234/1‑151 (MQ=21)
                                                      ggACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑AGATATTGCGCGCCCCGATGCCGACGATATTGCCTCCCCCGGCTGGAtag                                                                                                   >  2:1223731/1‑149 (MQ=21)
                                                      ggACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAAGTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑AAAGATGGCGCGCACCGATGCCGCCGATATGGCCACCCCCGGCTGGATTg                                                                                                   >  2:1223733/1‑151 (MQ=21)
                                                               gatcaggatAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAAt                                                                                            >  1:526152/1‑150 (MQ=18)
                                                               gatcaggatAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCACCCCCGGCTGGATTGAGAAAATCg                                                                                          >  1:44584/1‑151 (MQ=18)
                                                               gatcaggatAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGG‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCgg                                                                                         >  2:978896/1‑150 (MQ=255)
                                                                    ggatAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGTTTTAGAAAAACGTggg                                                                                      >  2:1130839/1‑151 (MQ=18)
                                                                    ggatAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTggg                                                                                      >  1:897814/1‑151 (MQ=18)
                                                                    ggatAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTggg                                                                                      >  1:1088865/1‑150 (MQ=255)
                                                                        aaTTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATAGTGGGCgag                                                                                  >  2:78747/1‑151 (MQ=17)
                                                                          tttCCAATCCCCGCAATTTGGGCTTTATCTCCTCTTTAAACATCGGGCTGGACGAATAGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGG‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGCCTGGATTGAGAAAATCGTGGGCGGGATgg                                                                              >  2:167888/1‑151 (MQ=17)
                                                                          tttCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGGGA‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCAACCCCCGGATGGATTGAGAAAATCGTGGGCgtta                                                                                 >  1:438377/1‑148 (MQ=17)
                                                                          tttCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAAAATCGGGCTGGACGAATTGGCAA‑‑AGTCTG‑‑GGGG‑‑‑‑GGTGGGG‑‑‑‑‑C‑‑‑‑ATATATAGCGCGCACCGATGCCGACAATATTGCCACCCCCGGATGGATGGAGAAAATAGTGGGCGAGATg                                                                               >  2:864420/1‑151 (MQ=17)
                                                                          tttCCAATCCACTCAATTTGGGCTTTATCGCCACTATAAACATCGGGCTGGACGAATTGGCCA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATAAATTGCGCGCACCGATCCCGACGCTATTGCCTCCCCCGGCTGGCTGGAGAAATTCGTGGGCgagttg                                                                               >  2:516778/1‑148 (MQ=9)
                                                                                              ggCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTCGGGTGGGG‑‑‑‑GG‑GGGG‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGAAATTGCCTCCCCCGGCTGGATTGAGAAAAACGTGGGCGAGATGGAAAAAAACCgcagcaaca                                                            >  1:172355/1‑148 (MQ=14)
                                                                                                                      gggCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGGGGGTGG‑GGGG‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAg                                    >  2:947466/1‑151 (MQ=11)
                                                                                                                      gggCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGG‑‑A‑‑‑‑ATATATTTAGCGCACCGATGAAGACGATATTGCCTACCCCGGCTGGAGTGAGAAAATGGTGGTCGTTTAGTAAATAGACCGCAGCATCATTGCGTTGGGCGAGTGGTTGGAAGt                                   >  2:666971/1‑151 (MQ=2)
                                                                                                                       ggCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGGTGTTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAATACCGCACCATCATTGCGAGGGGCGCGTGGTTGGAAGt                                   >  2:1287965/1‑151 (MQ=11)
                                                                                                                       ggCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGt                                   >  2:105685/1‑151 (MQ=11)
                                                                                                                       ggCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑AAATATTGCGCGCACCGATGCCGACGTTATTGCCACCCCCGGCTGGGTTGAGAAAATCGTGGGCGAGATGGAAAAAGACCCCAGCAACATTGCGATGGGCGCGTGGTTGGAAGt                                   >  2:105699/1‑151 (MQ=11)
                                                                                                                       ggCTGGACGAATAGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGGGGGG‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCACCCCCGGCTGGATTGAGAAAATCGTGGGCGATATGGAAAAAGACCGCAGCATAATTGCGATGGGCGCGTGGTTGGAAGt                                   >  2:622380/1‑151 (MQ=11)
                                                                                                                                         cccgcggggggggg‑‑‑‑gg‑ggcc‑‑‑‑‑A‑‑‑‑ATATAGTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAAc                   <  1:248269/141‑1 (MQ=11)
                                                                                                                                           agtcgggggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATAATTGCGAt                                                     >  1:577624/9‑115 (MQ=11)
                                                                                                                                           agtcgggggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATAAATTGCGCGCACCGTTGCCCACGTTATTGCCACCCCCGGATGGATTGATAAAATCGTGGGCGAGATGGAAAAATACCGCAGCATAATTGCGATGGGCGCGTGGTTGGAAGTTTTTTCGGAATaaaaaaa                 >  2:1197054/9‑148 (MQ=11)
                                                                                                                                             gtcggggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCCCCGATCCCGACGATATTGCCACCCCCGGATGGATTGTTAAAAACGTGGGCGAGATGGAAAAAGACCCCACCAACATTGCGATGGGCGCGTGGTTGGAATTTTTGTCGGAAGAAAACAATa               >  2:80137/7‑151 (MQ=11)
                                                                                                                                             gtcggggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACTATATTGCCTCCCCCGGCTGGATTGATAAAATCGTGGGCGATTTGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGGGGTTGGAAGTTTTTTCGGAAGAAAACAATa               >  2:80139/7‑151 (MQ=14)
                                                                                                                                             gtcggggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATa               >  1:860920/7‑151 (MQ=255)
                                                                                                                                                ggggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCAATGCCGACAAATTTGCCACCCCCGGAGGTATTGAGAAAATCGTGGGCGATATGTAAAAATACCGCAGCAAAAATGCGATGGGCGCGTGTTTGGAATTTTTTGCGGAATAAAACAATaaag            >  2:667522/4‑150 (MQ=2)
                                                                                                                                                  ggggg‑‑‑‑gg‑gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGTCGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAg           <  1:875303/149‑1 (MQ=255)
                                                                                                                                                  ggggg‑‑‑‑gg‑ggcc‑‑‑‑‑A‑‑‑‑ATATAGTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAg           <  1:117150/149‑1 (MQ=255)
                                                                                                                                                  ggggg‑‑‑‑gc‑gggg‑‑‑‑‑A‑‑‑‑ATAGATAGCGCGCACCGCTGCCGACGATCGTGCCTCCCCCGGCGGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGAGGGGCGCGTGGTTGGAAGTTTTGGCGGAAGAAAACAATAAAAg           <  2:1256925/149‑1 (MQ=14)
                                                                                                                                                    ggg‑‑‑‑gg‑ggcg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCg         <  2:413670/150‑1 (MQ=255)
                                                                                                                                                              gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTg   >  2:663795/1‑151 (MQ=255)
                                                                                                                                                              gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTg   >  2:637341/1‑151 (MQ=255)
                                                                                                                                                              gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTg   >  2:637342/1‑151 (MQ=255)
                                                                                                                                                              gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTg   >  2:637347/1‑151 (MQ=255)
                                                                                                                                                              gggg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTg   >  2:1024408/1‑151 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAg                                    >  2:722190/1‑116 (MQ=33)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAg                                    >  2:722213/1‑116 (MQ=33)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAg                                    <  1:722388/116‑1 (MQ=33)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAg                                    <  1:722411/116‑1 (MQ=33)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGACAACAATAAAAGCGTGCTTgc  >  1:1211630/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACACTAAAAGCGTGCTTgc  >  2:448111/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:920923/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:878684/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:1126688/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:621091/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:448105/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:323533/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:27919/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:142527/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:1312364/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:1142515/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:993235/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:906554/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:906550/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:888948/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:888909/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:1289517/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  1:1104429/1‑150 (MQ=255)
                                                                                                                                                                gg‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATGGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTgc  >  2:747960/1‑150 (MQ=255)
                                                                                                                                                                  ||                                                                                                                                                           
CGATGACGGCTCGACGGACGGCACGCCCGCCATTGCCCGGCGTTTCCAAGAACAGGACGGCAGGATCAGGATAATTTCCAATCCCCGCAATTTGGGCTTTATCGCCTCTTTAAACATCGGGCTGGACGAATTGGCAA‑‑AGTC‑‑‑‑GGGG‑‑‑‑GG‑GGGG‑‑‑‑‑A‑‑‑‑ATATATTGCGCGCACCGATGCCGACGATATTGCCTCCCCCGGCTGGATTGAGAAAATCGTGGGCGAGATGGAAAAAGACCGCAGCATCATTGCGATGGGCGCGTGGTTGGAAGTTTTGTCGGAAGAAAACAATAAAAGCGTGCTTGC  >  Exported/2135580‑2135878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑