Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Exported	42,301	5 bp→CCGGC	coding (156‑160/462 nt)	BCHEFKBB_00042 ←	acetyl‑CoA carboxylase [NGO_0045]

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	42,301	0	T	C	100.0%	68.6 / NA	18	T54A (ACC→GCC)	BCHEFKBB_00042	acetyl‑CoA carboxylase [NGO_0045]
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/1);  total (17/1)
*	Exported	42,302	0	A	C	100.0%	68.6 / NA	18	C53P (TGT→CCG)	BCHEFKBB_00042	acetyl‑CoA carboxylase [NGO_0045]
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/1);  total (17/1)
*	Exported	42,303	0	C	G	100.0%	66.9 / NA	18	C53P (TGT→CCG)	BCHEFKBB_00042	acetyl‑CoA carboxylase [NGO_0045]
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/1);  total (17/1)
*	Exported	42,304	0	A	G	100.0%	66.9 / NA	18	C53P (TGT→CCG)	BCHEFKBB_00042	acetyl‑CoA carboxylase [NGO_0045]
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/1);  total (17/1)
*	Exported	42,305	0	A	C	100.0%	68.6 / NA	18	A52A (GCT→GCG)	BCHEFKBB_00042	acetyl‑CoA carboxylase [NGO_0045]
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/1);  total (17/1)

GCGCGGCGGCGGGCGCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGTACAAGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCAACCAAAT  >  Exported/42250‑42429                                                    |||||                                                                                                                             gcgcggcgGCGGGCGCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGAt                               >  2:599170/1‑151 (MQ=255) gcgcggcgGCGGGCGCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGAt                               >  2:599168/1‑151 (MQ=255) gcgcggcgGCGGGCGCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGAt                               >  2:138186/1‑151 (MQ=255) gcgcggcgGCGGGCGCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGGACACGCGAGCTGCCGCAGCTGCAATGGTCCGGGTAATGCGGACTTGTTCCTCGCCATCGGTTACTTCGAt                               >  2:138187/1‑151 (MQ=255)            ggcgCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATa                     >  1:597958/1‑150 (MQ=255)            ggcgCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATa                     >  1:654091/1‑150 (MQ=255)              cgcgGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATAcc                   >  2:714200/1‑150 (MQ=255)                  gATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGAtt              >  2:1126995/1‑151 (MQ=255)                      ccGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGAttctt           >  1:852769/1‑150 (MQ=255)                      ccGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGAttc             <  2:899628/148‑1 (MQ=255)                      ccGCAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGAttc             >  1:899869/1‑148 (MQ=255)                      ccGCAACGGGTGCGACGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGAttctt           >  1:560147/1‑150 (MQ=255)                      ccGCAACGGGTGCGACGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGAttctt           >  1:560149/1‑150 (MQ=255)                         cAACGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCaa        >  2:1354070/1‑150 (MQ=255)                           aCGGGTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCAACCa     >  2:47871/1‑151 (MQ=255)                             gggTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCACTGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCAACCaaa   >  2:663817/1‑151 (MQ=255)                             gggTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCAACCaca   >  1:411424/1‑149 (MQ=255)                              ggTGCGGCGGCCGGCGTTACGGCCGGCGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCAACCAaat  >  1:574292/1‑151 (MQ=255)                                                    |||||                                                                                                                             GCGCGGCGGCGGGCGCGGATGCCGCAACGGGTGCGGCGGCCGGCGTTACGGTACAAGCGGCGGCAGGTACGGGCGCGGCGTAAACGGGAGCTGCCGCAGCTGCAATGGTACGGGTAATGCGGACTTTTTCCTCGCCTTCGGTTACTTCGATTTCGGCGATACCCGATTCTTCAACCAAAT  >  Exported/42250‑42429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑