breseq  version 0.37.1  revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 42,301 5 bp→5 bp coding (156‑160/462 nt) BCHEFKBB_00042 ← acetyl‑CoA carboxylase [NGO_0045]
RA 60,035 G→T G93C (GGC→TGC)  BCHEFKBB_00055 → MFS transporter [NGO_0059]
MC JC 358,371 (CGCAA)16→14 coding (128‑137/243 nt) BCHEFKBB_00366 → hypothetical protein [NGO_0366]
RA 522,455 A→G Q845R (CAG→CGG) 
T130T (ACT→ACC
BCHEFKBB_00548 →
BCHEFKBB_00549 ←
restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
RA 522,457 4 bp→CC coding (2536‑2539/2592 nt)
coding (385‑388/462 nt)
BCHEFKBB_00548 →
BCHEFKBB_00549 ←
restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
RA 522,467 2 bp→CGG coding (2546‑2547/2592 nt)
coding (377‑378/462 nt)
BCHEFKBB_00548 →
BCHEFKBB_00549 ←
restriction endonuclease EcoPI subunit R[NGO_0546]
hypothetical protein
RA 1,162,857 C→T G411D (GGC→GAC)  BCHEFKBB_01205 ← long‑chain fatty acid‑‑CoA ligase [NGO_1213]
RA 1,788,228 (C)10→11 intergenic (+767/‑103) BCHEFKBB_01823 → / → BCHEFKBB_01824 hypothetical protein [NGO_1810]/pseudouridine synthase [NGO_1811]
RA 2,073,047 (G)9→12 intergenic (‑108/‑396) BCHEFKBB_02124 ← / → BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
JC 2,134,653 (G)9→14 coding (166/183 nt) BCHEFKBB_02183 → hypothetical protein
RA 2,135,730 (G)10→12 intergenic (+323/‑69) BCHEFKBB_02184 → / → BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ Exported 745788–745878 747126–745880 3–1339 79 [76] [71] 78 BCHEFKBB_00758–[BCHEFKBB_00760] BCHEFKBB_00758,BCHEFKBB_00759,[BCHEFKBB_00760]
* * ÷ Exported 1072607–1075092 1077570–1075129 38–4964 79 [69] [69] 86 [BCHEFKBB_01101]–BCHEFKBB_01111 [BCHEFKBB_01101],BCHEFKBB_01102,BCHEFKBB_01103,BCHEFKBB_01104,BCHEFKBB_01105,BCHEFKBB_01106,BCHEFKBB_01107,BCHEFKBB_01108,BCHEFKBB_01109,BCHEFKBB_01110,BCHEFKBB_01111
* * ÷ Exported 1160013–1160223 1160519–1160229 7–507 89 [46] [74] 77 [BCHEFKBB_01202]–[BCHEFKBB_01203] [BCHEFKBB_01202],[BCHEFKBB_01203]
* * ÷ Exported 1215620–1223339 1223486–1223386 48–7867 82 [76] [76] 77 BCHEFKBB_01257–BCHEFKBB_01268 BCHEFKBB_01257,BCHEFKBB_01258,BCHEFKBB_01259,BCHEFKBB_01260,BCHEFKBB_01261,BCHEFKBB_01262,BCHEFKBB_01263,BCHEFKBB_01264,BCHEFKBB_01265,BCHEFKBB_01266,BCHEFKBB_01267,BCHEFKBB_01268
* * ÷ Exported 2065363–2065503 2065919–2065621 119–557 82 [75] [74] 77 [BCHEFKBB_02118] [BCHEFKBB_02118]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported = 75346NA (NA)184 (1.510) 36/214 0.7 NA intergenic (‑87/‑1007) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported = 1482447 NA (NA)intergenic (+392/‑83) BCHEFKBB_01513/BCHEFKBB_01514 hypothetical protein [NGO_07995]/Opacity protein opA54 [NGO_1513]
* ? Exported 1886992 =167 (1.010)48 (0.410)
+43 bp
23/206 2.0 29.3% intergenic (+141/+341) BCHEFKBB_01930/BCHEFKBB_01931 membrane protein [NGO_1910]/GIY‑YIG nuclease [NGO_1911]
?Exported = 1888024 161 (0.970)intergenic (‑407/+174) BCHEFKBB_01931/BCHEFKBB_01932 GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]