breseq  version 0.37.1  revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 47 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,135,7303.G79.1% 81.3 / 32.1 43intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
*Exported1,729,7181.G75.8% 164.2 / 55.6 64coding (745/753 nt)BCHEFKBB_01772glycosyl transferase family 1 [NGO_1765]
*Exported2,073,0474.G66.7% 45.2 / 31.1 27intergenic (‑108/‑396)BCHEFKBB_02124/BCHEFKBB_02125FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
*Exported2,135,7304.G55.8% 52.9 / 77.7 43intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
*Exported55,5910TA43.6% 24.6 / 11.7 39N36K (AAT→AAABCHEFKBB_00050hypothetical protein
*Exported1,831,9900GA35.5% 214.9 / 225.6 206intergenic (‑123/+230)BCHEFKBB_01881/BCHEFKBB_01882Opacity protein opA54 [NGO_09965]/transposase [NGO_09970]
*Exported2,015,0140GC35.4% 494.1 / inf 261Q158E (CAA→GAA) BCHEFKBB_02064large pilS cassette protein [NGO_10975]
*Exported1,887,9340TC33.0% 359.5 / 233.9 228intergenic (‑317/+264)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported2,015,0080CT32.9% 496.5 / 269.7 257A160T (GCT→ACT) ‡BCHEFKBB_02064large pilS cassette protein [NGO_10975]
*Exported1,891,0250AT32.8% 192.1 / 23.9 116L75Q (CTG→CAG) BCHEFKBB_01932pilus assembly protein [NGO_10270]
*Exported2,015,0010GT31.5% 536.6 / 271.5 271T162N (ACC→AAC) ‡BCHEFKBB_02064large pilS cassette protein [NGO_10975]
*Exported2,015,0020TC30.9% 563.7 / 271.7 276T162A (ACC→GCC) ‡BCHEFKBB_02064large pilS cassette protein [NGO_10975]
*Exported2,015,0060AT30.9% 547.0 / 250.6 275A160A (GCT→GCA) ‡BCHEFKBB_02064large pilS cassette protein [NGO_10975]
*Exported1,887,9430AG30.7% 399.5 / 221.5 246intergenic (‑326/+255)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported2,015,0070GT30.6% 553.3 / 269.1 275A160D (GCT→GAT) ‡BCHEFKBB_02064large pilS cassette protein [NGO_10975]
*Exported101,5870AT30.5% 320.4 / 38.1 170L111Q (CTG→CAG) BCHEFKBB_00090shikimate kinase [NGO_0093]
*Exported1,887,9410CA28.3% 451.8 / 192.8 248intergenic (‑324/+257)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported999,0790GT26.8% 307.4 / 20.1 159Y7* (TAC→TAABCHEFKBB_01021hypothetical protein
*Exported1,690,8550TG25.5% 281.5 / 14.8 148A57A (GCT→GCGBCHEFKBB_01736peptidase [NGO_1729]
*Exported801,4600CA25.1% 365.8 / 28.2 179R178R (CGC→CGAcysISulfite reductase [NADPH] hemoproteinbeta‑component

Marginal new junction evidence (lowest skew 10 of 86 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported 2132863 =118 (0.710)32 (0.230)
+TCCCCCCCCCCCCCC
20/242 3.5 32.9% intergenic (+329/‑67) BCHEFKBB_02180/BCHEFKBB_02181 glycyl‑tRNA synthetase subunit beta [NGO_2154]/glycosyl transferase [NGO_11610]
?Exported = 2135720 37 (0.240)intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported = 51928106 (0.640)38 (0.250) 23/268 3.5 23.6% intergenic (‑429/+96) BCHEFKBB_00048/BCHEFKBB_00049 carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055]
?Exported 1887832 = 148 (0.970)intergenic (‑215/+366) BCHEFKBB_01931/BCHEFKBB_01932 GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
* ? Exported = 213572016 (0.100)53 (0.330)
+GGGG
22/264 3.6 76.7% intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
?Exported 2135721 = 16 (0.100)intergenic (+314/‑78) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported = 20730385 (0.030)40 (0.250)
+GGGG
17/266 4.9 88.8% intergenic (‑99/‑405) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
?Exported 2073039 = 5 (0.030)intergenic (‑100/‑404) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
* ? Exported = 75346163 (0.980)20 (0.170) 11/204 5.1 16.1% intergenic (‑87/‑1007) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported 1000281 = 94 (0.810)intergenic (‑108/‑398) BCHEFKBB_01022/BCHEFKBB_01023 Opacity protein opA54 [NGO_05420]/hypothetical protein [NGO_1040]
* ? Exported = 2015238332 (2.000)13 (0.100) 12/230 5.5 5.2% coding (248/681 nt) BCHEFKBB_02064 large pilS cassette protein [NGO_10975]
?Exported 2015627 = 213 (1.630)coding (313/363 nt) BCHEFKBB_02065 hypothetical protein [NGO_10980]
* ? Exported 2015596 =276 (1.660)13 (0.100) 11/230 5.9 6.9% coding (344/363 nt) BCHEFKBB_02065 hypothetical protein [NGO_10980]
?Exported = 2017675 136 (1.040)coding (282/483 nt) BCHEFKBB_02068 fimbrial protein [NGO_10995]
* ? Exported 1231241 =124 (0.750)56 (0.400) 11/244 6.3 35.1% intergenic (+682/‑102) BCHEFKBB_01273/BCHEFKBB_01274 hypothetical protein [NGO_1277]/Opacity protein opA54 [NGO_06725]
?Exported = 1482462 NA (NA)intergenic (+407/‑68) BCHEFKBB_01513/BCHEFKBB_01514 hypothetical protein [NGO_07995]/Opacity protein opA54 [NGO_1513]
* ? Exported = 2039437140 (0.840)9 (0.070) 9/222 6.5 7.6% intergenic (‑477/+339) pilE/BCHEFKBB_02088 Fimbrial protein/pilin [NGO_11115]
?Exported 2039830 = 111 (0.880)coding (369/423 nt) BCHEFKBB_02088 pilin [NGO_11115]
* ? Exported = 75312NA (NA)32 (0.250) 9/224 6.5 35.4% intergenic (‑53/‑1041) BCHEFKBB_00065/BCHEFKBB_00066 Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
?Exported = 924765 76 (0.460)intergenic (‑516/‑44) BCHEFKBB_00942/BCHEFKBB_00943 proline iminopeptidase [NGO_0949]/Opacity protein opA54 [NGO_08230]