Predicted mutation
evidence seq id position mutation annotation gene description
RA Exported 1,788,228 (C)10→11 intergenic (+767/‑103) BCHEFKBB_01823 → / → BCHEFKBB_01824 hypothetical protein [NGO_1810]/pseudouridine synthase [NGO_1811]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,788,2281.C82.1% 215.2 / 45.2 67intergenic (+767/‑103)BCHEFKBB_01823/BCHEFKBB_01824hypothetical protein [NGO_1810]/pseudouridine synthase [NGO_1811]
Reads supporting (aligned to +/- strand):  ref base . (1/11);  new base C (0/55);  total (1/66)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CTGTCGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTACGA  >  Exported/1788079‑1788377
                                                                                                                                                         |                                                                                                                                                       
cTGTCGGTGCGGAAACTTTTCGGATAAATTGGTTTCTTTAGTTTTTTACGTCCTATATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGCCAGGGTTAATTCAAAGGATAGCGATTCGTCCCAACTGCATCC‑AA‑c‑cccccccccc                                                                                                                                                         <  2:276280/151‑2 (MQ=255)
    cGGTGCGGAAACTTTTCGGATAAAATGGTTTCTTTTTATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGTATTCACAGGATAGCGTTTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  2:1230768/151‑6 (MQ=38)
    cGGTGCGGAAACTTATCGTATAAAATGGTTTCTTTATATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  2:1230760/151‑6 (MQ=255)
    cGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTCGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  1:1445465/151‑6 (MQ=255)
    cGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACCGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  1:1445454/151‑6 (MQ=255)
    cGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  1:90454/151‑6 (MQ=255)
    cGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  2:276561/151‑6 (MQ=255)
    cGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  1:308302/151‑6 (MQ=255)
    cGGTGCGCAAACTTACCGGATAAAATTGTTTCATTAGATATTTACGACCTAGATTCCCGCCTGCGCGGGAATCACGGTTCGGGCATTCCAGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCAACC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  2:680853/151‑6 (MQ=255)
     ggTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑ccccccccccgaac                                                                                                                                                     <  1:704921/150‑6 (MQ=255)
      gTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑cccccccccccgaac                                                                                                                                                    <  1:1567313/150‑7 (MQ=255)
      gTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑cccccccccccgaac                                                                                                                                                    <  1:1419522/150‑7 (MQ=255)
      gTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑cccccccccccgaac                                                                                                                                                    <  1:1419494/150‑7 (MQ=255)
      gTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑c‑cccccacccccaacca                                                                                                                                                   >  2:854988/1‑144 (MQ=255)
      gTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑A‑‑C‑CCcccgccc‑‑‑gcgagcaa                                                                                                                                               >  2:1258241/1‑144 (MQ=38)
       tGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑Gaacaac                                                                                                                                                <  2:166/151‑1 (MQ=255)
          ggaaaagtATCGGATAAAATGGTTTCTTTATATTTTTACGTCCTATATTCCCGCCTGCGCGGGATTTACGGTTCGGGCACTCCTGACAGGGGTCATTCAAAGGATTGCGATTCGTTGCAACTGCATAC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTc                                                                                                                                              <  2:545478/143‑1 (MQ=255)
                ttATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAaac                                                                                                                                       <  1:873867/151‑1 (MQ=255)
                   tCGGATAAAATTGTTTATTTTGTTTTTTACGTCCTTTTTTCCCGCCTGGGGGGGAATGTAGGTTCGGGCACTCCTTAAAGGGTGAATTCACAGGTTTGCGTTTAGTAGCAAATGCATCC‑AA‑C‑CCCCCCCCCCCCGAACAACTCCCCAaaca                                                                                                                                      <  1:1912091/151‑1 (MQ=255)
                          aaaTGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAaaca                                                                                                                                      <  2:1663981/142‑1 (MQ=255)
                                   cTTTAGATTGTTACGTCCGAGAGTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGACGTCACAGGATAGCGATTCGTAGCAACTGCCTCC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACACCGCCGCGCGCCCCGGGCg                                                                                                                   <  2:1787526/151‑1 (MQ=255)
                                    tttATATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCg                                                                                                                   <  2:1636154/151‑1 (MQ=255)
                                    tttAGATTTTTACGTCCTTGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCg                                                                                                                   <  1:1434579/151‑1 (MQ=255)
                                    tttAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACCGGGTGAATTCACCGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACCACGCCGCGCGCCCCGGGCg                                                                                                                   <  1:80344/151‑1 (MQ=255)
                                    tttAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGTATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCg                                                                                                                   <  2:1426982/151‑1 (MQ=255)
                                    tttAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCg                                                                                                                   <  1:1484246/151‑1 (MQ=255)
                                    tttAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCg                                                                                                                   <  1:1721063/151‑1 (MQ=255)
                                     ttcgATTTTTACGTCCTCCCTTCCCGCCGGCGCGGGAATGACGGTTCGGGCACTCCTGCAAGGGTGACTTCACAGGCTAGCGATGAGCACCAACTGCATCC‑AA‑C‑CCCCCCCCCCC‑GACCCACTCCCCCAACAACGCCGCGCGCCCCGGGCg                                                                                                                   <  2:1921653/148‑1 (MQ=255)
                                                                   cgcgGGTATGTCGGTTCGGGCAATCCTGTCATGGTGTATTCAAAGGTTAGCGTTTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATAc                                                                                     <  2:1079788/150‑1 (MQ=255)
                                                                   cgcgGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATAc                                                                                     <  2:181770/150‑1 (MQ=255)
                                                                   cgcgGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATAc                                                                                     <  1:1694675/150‑1 (MQ=255)
                                                                     cgGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACaa                                                                                   <  1:401119/150‑1 (MQ=255)
                                                                     cgGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACaa                                                                                   <  1:401134/150‑1 (MQ=255)
                                                                       ggAATGACGGTTCGGGCACTCCTGACTGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCC‑‑‑GACCAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGc                                                                                <  2:262599/150‑1 (MQ=255)
                                                                        taattacgtttCGGGCAATCCTTAAAGGGTTAATTAACAGGAAACCTTTTCGTACCAACTGCAACC‑AA‑C‑CCCCCCCCCCC‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGc                                                                                <  1:1301811/142‑1 (MQ=255)
                                                                        taaTGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCTCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGc                                                                                <  2:68872/149‑1 (MQ=255)
                                                                        gAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGc                                                                                <  1:183713/150‑1 (MQ=255)
                                                                        gAATGACGGTTCGGGCAATCCTGACAGGGTGAATTCACAGGATTGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGc                                                                                <  1:464778/150‑1 (MQ=255)
                                                                         atttaCGGTTCGGGCACTCCTGCCAGGGTGAATTCACAGGTTAGCTATTCTTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCCC‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGc                                                                                <  2:799029/146‑1 (MQ=255)
                                                                         aatgtcgtttCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCTTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACACCGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAg                                                                              <  2:1688704/143‑1 (MQ=255)
                                                                         aaTGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAg                                                                              <  1:1749513/151‑1 (MQ=255)
                                                                         aaTGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACCGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAg                                                                              <  1:1625429/151‑1 (MQ=255)
                                                                                 ttCGGGCACTCCTGACAGGGTGAATTCACAGTATAGCGATTCGTAGCAACTGCATCCTAC‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAAct                                                                      <  2:1255707/151‑2 (MQ=255)
                                                                                 ttCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACa                                                                      <  1:1175517/151‑1 (MQ=255)
                                                                                 ttCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACa                                                                      <  1:668551/151‑1 (MQ=255)
                                                                                        aCTCCTGACAGGGTGAATTAAAAGGATAGCGATTCGTAGCAACTGCATCC‑AAAC‑CCCCCCCCC‑‑‑GAACAACTCCCCCACCAACGCCGAGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATcc                                                               <  2:185530/151‑1 (MQ=255)
                                                                                             tGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTc                                                                                                                                              <  1:888630/67‑1 (MQ=255)
                                                                                                    gTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAAcc                                                   <  2:609508/150‑1 (MQ=255)
                                                                                                     tGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACACCGCCGCGCGCCCCGGGCGTTTGCCGTTACCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCACAcc                                                   <  2:1462980/150‑1 (MQ=255)
                                                                                                     tGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAAcc                                                   <  1:1203438/150‑1 (MQ=255)
                                                                                                     tGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAAccc                                                  <  1:1314385/150‑1 (MQ=255)
                                                                                                           cacaGGTTAGCGATTCGTAGCAACTGCATCC‑AC‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAATCACCGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTCCAAAATCTGAGATAGTAGTCAGTCTGAACATTTATCCGAATCCCAAACCCGATggt                                            <  2:1744106/151‑2 (MQ=255)
                                                                                                           caaaGGATAGCGAGTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTGTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGa                                            <  1:1582762/148‑1 (MQ=255)
                                                                                                               ggATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATAc                                         <  2:385596/149‑1 (MQ=255)
                                                                                                                gATAGCGATTCGTAGCAACTTCATCC‑AA‑CACCCCCCCCC‑‑‑GAACATCTCCCCAAACAACGCCGCGCGCCCCTGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATAc                                         <  2:1570961/149‑1 (MQ=255)
                                                                                                                gATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAACCAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGAt                                           <  1:1480049/147‑1 (MQ=255)
                                                                                                                gATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATAcc                                        <  1:409943/150‑1 (MQ=255)
                                                                                                                gATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATAc                                         <  1:872866/149‑1 (MQ=255)
                                                                                                                gATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATAc                                         <  1:1847043/149‑1 (MQ=255)
                                                                                                                 aTAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGc                                      <  1:1189914/151‑1 (MQ=255)
                                                                                                                       aTTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACaaa                                 <  1:843532/150‑1 (MQ=255)
                                                                                                                         tCGTAGCAACTGCAACC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAaac                              <  2:120125/151‑1 (MQ=255)
                                                                                                                               caccggcacccacccc‑ccccccccc‑‑‑GAACAACTCCCCAACCAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGc                         <  2:1566657/136‑1 (MQ=255)
                                                                                                                               cAACGGCATCC‑AA‑C‑CCCCCCCCCC‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAAACTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGc                         <  1:1684057/150‑1 (MQ=255)
                                                                                                                                  cTGCAACC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCACACACCGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGc                    <  2:1675573/151‑1 (MQ=255)
                                                                                                                                          caacc‑ccccccccc‑‑‑TAACATCTCCACAAAATTCGCCGCGCGGCCGGGGCGTTTGCCGTTTCCCTGCAAAATATGCGATACTATGCAGTCTGAACATTTTTCCGATTCACAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAAc              <  1:737240/147‑1 (MQ=255)
                                                                                                                                           aacc‑ccccccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCAGTTTCCCTGCAAAATCTGCGATACATTGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAAcc             <  2:62825/148‑1 (MQ=255)
                                                                                                                                            ccc‑ccccccccc‑‑‑GAACACCTCCCCAAACCACGCCGCGCGCCCCGGGCGTTTGCAGTTTCCCTGCAAAATCTGCGATACAATCCAGTCTGAACATTAAACCGAATCCCCAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAAccc            <  2:1819818/149‑1 (MQ=255)
                                                                                                                                            acc‑ccccccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAAccc            <  1:1234529/149‑1 (MQ=255)
                                                                                                                                            acc‑ccccccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAAccc            <  2:1350737/149‑1 (MQ=255)
                                                                                                                                            acc‑ccccccccc‑‑‑GAACAACTCCCCAAACAACCCCGCGCGCCCCGGCCGTTTGCCGTTTCCATGCAAAATCTGCGATACAATGCAGACTCCACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACACCGCTGGGCAATAAccc            <  2:1819830/149‑1 (MQ=255)
                                                                                                                                            acc‑ccccccccc‑‑‑GAACAACGCCCCAAACAACGCCGCGCGCCCCGGGCGTTGGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAAccc            <  2:1045042/149‑1 (MQ=255)
                                                                                                                                                  ccccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAACCCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATcc       <  2:820651/151‑1 (MQ=255)
                                                                                                                                                  ccccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAACAACAACGCTGGGCAATAACCCTATcc       <  2:364176/151‑1 (MQ=255)
                                                                                                                                                  ccccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATcc       <  1:11644/151‑1 (MQ=255)
                                                                                                                                                   cccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCAGCAAAATCAGCGATACAAAGCAGTCTGAACATTTATCCGAATACCAAAACCGATGGATACCGCACAAAAACAACGCAGGGCAATAACCCTATc        >  2:1455793/1‑149 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTa     <  1:1060361/151‑1 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTa     <  1:1060350/151‑1 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATAAAATGCAGTGTGTACATTTATCCGAATACCAAACCCCATGGATAACGCACAAAAAAAACGCTGGGCAAAAACCCTATACTa     >  1:1109820/1‑151 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATATGCGATACAATGCAGTCTGAACAATTATACGAATACCAAACCCGATTGATACCGCACAAAAACAACGCTGGGCAATAACCCAATCCTa     >  2:1060002/1‑151 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCGGCAAAATATGCGATAAAATGCAGTCTGAACATTTATACGAATACCAAAACCCATGGATACCGCACAAAAACAAAGCTGGGCCATAACCCAATccaa     >  1:476282/1‑149 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCAGCAAAATCTGCGATACAATGCAGTCTGAACATTTATACGAATCCCAAACCCCATGGATAACGCACAAAAACAACGCTGGGCAATAACCCAATCCTa     >  1:1405042/1‑151 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTACCTGCAAAATCTGCGATAAAATGCAGTCTGAACAATTATACGAATCCCAAACCCCATGGATAACGCACAAAAACAACGCTGGGCAAAAACCCTATCCTa     >  2:1059991/1‑151 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTACCTGCAAAAACTGCGATACAATGCAGTCTGAACAATTATACGAATCCCAAACCCCATGGATACCGCACCAAAACAACGCTGGGCCATAACCCAATCCTa     >  1:1761620/1‑151 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTGTGCCGTTTCCCTGCAAAATCTGCGATACAATTCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACCCTGGGCAATAACCCTATCCTa     >  1:476293/1‑151 (MQ=255)
                                                                                                                                                    ccccc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTGTGCCGTTTACCTGCAAAATCTGCGATACAATGCAGTCTGAACAATTATCCGAATCCCAAACCCGATGGATAACGCACAAAAAAAAAGCTGGGCAATAACCCAATCCTa     >  2:1060072/1‑151 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCCAACCCCGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCAGCAAAATCTGCGATACAATGCAGTCTGACCATTTACCCGAATCCCAAACCCGATGGATACCGCACAAAAACCCCGCTGGGCAAGAACCCTATCcgacga  <  2:1140480/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAACCACCGCCGCGCGCCCCGCGCTTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGCTGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:304002/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACCACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:1046897/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTTAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:304038/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  2:113387/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:859135/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  2:1065673/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  2:1065696/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  2:394738/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:1728490/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  2:283235/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  2:113391/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:1510312/151‑1 (MQ=255)
                                                                                                                                                       cc‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTacga  <  1:1510377/151‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                       
CTGTCGGTGCGGAAACTTATCGGATAAAATGGTTTCTTTAGATTTTTACGTCCTAGATTCCCGCCTGCGCGGGAATGACGGTTCGGGCACTCCTGACAGGGTGAATTCACAGGATAGCGATTCGTAGCAACTGCATCC‑AA‑C‑CCCCCCCCC‑‑‑GAACAACTCCCCAAACAACGCCGCGCGCCCCGGGCGTTTGCCGTTTCCCTGCAAAATCTGCGATACAATGCAGTCTGAACATTTATCCGAATCCCAAACCCGATGGATACCGCACAAAAACAACGCTGGGCAATAACCCTATCCTACGA  >  Exported/1788079‑1788377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑