BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Exported	522,457	4 bp→CC	coding (2536‑2539/2592 nt) coding (385‑388/462 nt)	BCHEFKBB_00548 → BCHEFKBB_00549 ←	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	522,457	0	T	.	99.0%	512.3 / ‑3.0	103	coding (2536/2592 nt) coding (388/462 nt)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (43/59); total (44/59)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	Exported	522,458	0	A	.	99.0%	514.4 / ‑1.4	103	coding (2537/2592 nt) coding (387/462 nt)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (43/59); minor base G (1/0); total (44/59)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-02
*	Exported	522,459	0	T	C	100.0%	411.2 / NA	103	Y846Y (TAT→TAC) Y129R (TAT→CGT)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (44/59); total (44/59)
*	Exported	522,460	0	A	C	100.0%	420.2 / NA	103	M847L (ATG→CTG) Y129R (TAT→CGT)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (44/59); total (44/59)

Only 100 of 103 total aligned reads displayed.

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAAT  >  Exported/522342‑522582

||

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCACATTTACAAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGAAGATTTGAAGATACGGAGCACC‑‑TGCCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:239895/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:1059199/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:1059235/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:1479009/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:1479013/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:1588818/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:1639899/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  1:3149/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  1:1753216/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  1:1555051/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                            >  2:470469/1‑150 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAGAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                           <  1:1084757/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                           <  1:1084756/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                           <  1:33255/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                           <  1:1110555/150‑1 (MQ=255)

      ccGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCg                                                                                     >  2:1396196/1‑151 (MQ=255)

           ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                                <  1:1273825/151‑1 (MQ=255)

           ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                                <  2:1947051/151‑1 (MQ=255)

           ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                                <  2:813217/151‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGACGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                              <  1:1389465/150‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                              <  1:1155493/150‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                              <  1:1650587/150‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGa                                                                             <  1:1346131/151‑1 (MQ=255)

               cgtcagcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTAGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  2:229160/148‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCACATTTACCAATAACCCCGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGACGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  2:630698/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAATGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  1:1169937/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  2:229176/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  1:709893/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  1:1559302/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                            <  2:866730/151‑1 (MQ=255)

                 gcatcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAACCCGCGCGCGGAGAAAACAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAAc                                                                           <  2:1793372/146‑1 (MQ=255)

                   agcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATATTCTACGATTCGGATATAGAACATg                                                                        >  2:220541/1‑151 (MQ=255)

                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                       >  1:170010/1‑151 (MQ=255)

                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                       >  1:170013/1‑151 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                      <  2:1529651/151‑1 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  2:492054/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  2:492071/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  1:1631385/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  1:416456/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  1:416418/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  2:735973/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  1:291365/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                    >  1:1944185/1‑151 (MQ=255)

                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                           >  1:614693/1‑151 (MQ=255)

                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                           >  1:614887/1‑151 (MQ=255)

                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                       >  2:1891350/1‑150 (MQ=255)

                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                       >  1:1800429/1‑150 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGACAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                  <  1:627655/151‑1 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                  <  2:1728761/151‑1 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                  <  1:926851/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                <  2:1443271/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                <  2:263477/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                <  1:963550/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                <  1:963551/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                <  1:1694361/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                <  1:1694303/151‑1 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                               >  2:1790200/1‑151 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                               >  2:891111/1‑151 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                               >  1:22977/1‑151 (MQ=255)

                                               aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                            <  2:341569/151‑1 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGACAATGTCGCCAACAACCAAATTGAAGGCg                                          >  2:832421/1‑151 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                          >  1:1837763/1‑151 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                          >  2:1809928/1‑151 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                          <  1:1491046/151‑1 (MQ=255)

                                                    gACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTaa                                       >  2:1279726/1‑151 (MQ=255)

                                                      cGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAAt                                      >  2:277199/1‑150 (MQ=255)

                                                       ggCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    >  1:1053048/1‑151 (MQ=255)

                                                        gcataCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  2:200745/146‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAACAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  2:1830390/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  2:623527/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  1:1710401/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  2:1804096/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  1:632791/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                    <  1:736212/150‑1 (MQ=255)

                                                          aaaCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                 <  2:1357193/151‑1 (MQ=255)

                                                          aaaCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                 <  1:1122724/151‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                            <  1:1347614/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                            <  1:1640459/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                            <  1:160637/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                            <  1:1347620/150‑1 (MQ=255)

                                                                    cgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAAt                       <  2:1789541/151‑1 (MQ=255)

                                                                       tGAAAACAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                     <  1:1610994/150‑1 (MQ=255)

                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGAGACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                     <  1:610780/150‑1 (MQ=255)

                                                                       tGAAAAAAGCAGATTTGGGCAGAATTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                     <  2:1686616/150‑1 (MQ=255)

                                                                        gAAAAAAGCAGATTTGGGCAGGTTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                   >  2:909382/1‑151 (MQ=255)

                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                   >  1:738763/1‑151 (MQ=255)

                                                                           aaaaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCgaga                <  1:1761613/151‑1 (MQ=255)

                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa              >  1:349420/1‑151 (MQ=255)

                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa              >  2:1410186/1‑151 (MQ=255)

                                                                              cgCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc             <  2:585298/150‑1 (MQ=255)

                                                                              cgCAGATGTGGGCAGATTTGAAGAAACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACACCCAAATTGAAGGCGTAAACGTATTTACCAAAATACCGAGAAAc             <  2:585297/150‑1 (MQ=255)

                                                                                cAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc             <  1:1268452/149‑1 (MQ=255)

                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGTATATAGACCATGACATTGTCGCCAACAACCAAATTGAAGGCGTAATCGTATGTACCAAAATACCGAGACACTCCa         >  2:590310/1‑151 (MQ=255)

                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATCTAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa         >  2:887026/1‑151 (MQ=255)

                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa         >  2:1756175/1‑151 (MQ=255)

                                                                                   aTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTc           <  1:1204864/148‑1 (MQ=255)

                                                                                      tGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGACCCTGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa     <  2:1375602/151‑1 (MQ=255)

                                                                                         gCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaaaa   <  1:806713/150‑1 (MQ=255)

                                                                                         gCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaaaa   <  1:1233455/150‑1 (MQ=255)

                                                                                          cAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAAt  <  2:1899596/150‑1 (MQ=255)

||

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAAT  >  Exported/522342‑522582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`