BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Exported	522,467	2 bp→CGG	coding (2546‑2547/2592 nt) coding (377‑378/462 nt)	BCHEFKBB_00548 → BCHEFKBB_00549 ←	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	522,466	1	.	C	100.0%	416.8 / NA	103	L849? (CTA→NGG) A127? (GCG→NCG)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (44/59); total (44/59)
*	Exported	522,467	0	T	G	100.0%	420.2 / NA	103	L849? (CTA→NGG) V126G (GTA→GGC)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (44/59); total (44/59)
*	Exported	522,468	0	A	G	100.0%	419.0 / NA	103	L849? (CTA→NGG) V126G (GTA→GGC)	BCHEFKBB_00548 BCHEFKBB_00549	restriction endonuclease EcoPI subunit R[NGO_0546] hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/59); total (44/59)

Only 100 of 139 total aligned reads displayed.

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACTT  >  Exported/522342‑522618

|||

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCACATTTACAAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGAAGATTTGAAGATACGGAGCACC‑‑TGCCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:239895/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:3149/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:1059199/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:1479009/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:1479013/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:1588818/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:1639899/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  1:1753216/1‑150 (MQ=255)

ggATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTa                                                                                                                                >  2:470469/1‑150 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAGAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                                                               <  1:1084757/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                                                               <  1:1084756/150‑1 (MQ=255)

 gATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTAc                                                                                                                               <  1:1110555/150‑1 (MQ=255)

      ccGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCg                                                                                                                         >  2:1396196/1‑151 (MQ=255)

           ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                                                                    <  2:813217/151‑1 (MQ=255)

           ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                                                                    <  1:1273825/151‑1 (MQ=255)

           ttGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGatat                                                                                                                    <  2:1947051/151‑1 (MQ=255)

              tCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAg                                                                                                                  <  1:1650587/150‑1 (MQ=255)

               cgtcagcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTAGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  2:229160/148‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCACATTTACCAATAACCCCGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGACGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  2:630698/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAATGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  1:1169937/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  1:709893/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  2:866730/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  1:1559302/151‑1 (MQ=255)

               cGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGaa                                                                                                                <  2:229176/151‑1 (MQ=255)

                   agcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATATTCTACGATTCGGATATAGAACATg                                                                                                            >  2:220541/1‑151 (MQ=255)

                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                                                           >  1:170010/1‑151 (MQ=255)

                    gcaAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGa                                                                                                           >  1:170013/1‑151 (MQ=255)

                     caAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaa                                                                                                          <  1:25259/151‑1 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                                                        >  2:492054/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                                                        >  1:291365/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                                                        >  2:735973/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                                                        >  1:416456/1‑151 (MQ=255)

                       aaaTCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGaaaa                                                                                                        >  1:1631385/1‑151 (MQ=255)

                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                                                               >  1:614693/1‑151 (MQ=255)

                                cGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCcaa                                                                                               >  1:614887/1‑151 (MQ=255)

                                     aaaTTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCaacaac                                                                                           >  2:1891350/1‑150 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                                                      <  2:1728761/151‑1 (MQ=255)

                                         ttACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAAt                                                                                      <  1:926851/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                                                    <  2:1443271/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                                                    <  1:963551/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                                                    <  2:263477/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                                                    <  1:963550/151‑1 (MQ=255)

                                           aCCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTg                                                                                    <  1:1694361/151‑1 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                                                                   >  1:22977/1‑151 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                                                                   >  2:891111/1‑151 (MQ=255)

                                            ccAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGa                                                                                   >  2:1790200/1‑151 (MQ=255)

                                               aTAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAgg                                                                                <  2:341569/151‑1 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGACAATGTCGCCAACAACCAAATTGAAGGCg                                                                              >  2:832421/1‑151 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                                                              >  1:1837763/1‑151 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                                                              <  1:1491046/151‑1 (MQ=255)

                                                 aaaGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCg                                                                              >  2:1809928/1‑151 (MQ=255)

                                                    gACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTaa                                                                           >  2:1279726/1‑151 (MQ=255)

                                                       ggCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        >  1:1053048/1‑151 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        <  2:1804096/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        <  1:632791/150‑1 (MQ=255)

                                                        gCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCg                                                                        <  1:736212/150‑1 (MQ=255)

                                                          aaaCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                                                     <  1:1122724/151‑1 (MQ=255)

                                                          aaaCCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTAt                                                                     <  2:1357193/151‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                                                                <  1:1640459/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                                                                <  1:1347614/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                                                                <  1:160637/150‑1 (MQ=255)

                                                                cgcgcgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTAcc                                                                <  1:1347620/150‑1 (MQ=255)

                                                                    cgGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAAt                                                           <  2:1789541/151‑1 (MQ=255)

                                                                       tGAAAAAAGCAGATTTGGGCAGATTTGAAGAGACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATAc                                                         <  1:610780/150‑1 (MQ=255)

                                                                        gAAAAAAGCAGATTTGGGCAGGTTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                                                       >  2:909382/1‑151 (MQ=255)

                                                                        gAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCg                                                       >  1:738763/1‑151 (MQ=255)

                                                                           aaaaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCgaga                                                    <  1:1761613/151‑1 (MQ=255)

                                                                             aaGCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGaaa                                                  >  1:349420/1‑151 (MQ=255)

                                                                              cgCAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc                                                 <  2:585298/150‑1 (MQ=255)

                                                                              cgCAGATGTGGGCAGATTTGAAGAAACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACACCCAAATTGAAGGCGTAAACGTATTTACCAAAATACCGAGAAAc                                                 <  2:585297/150‑1 (MQ=255)

                                                                                cAGATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAAc                                                 <  1:1268452/149‑1 (MQ=255)

                                                                                  gATTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCa                                             >  2:1756175/1‑151 (MQ=255)

                                                                                   aTTTGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTc                                               <  1:1204864/148‑1 (MQ=255)

                                                                                      tGGGCAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGACCCTGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCaa                                         <  2:1375602/151‑1 (MQ=255)

                                                                                          cAGATTTGAAGATACGGAGCACCGG‑‑CCTGCCGCCGGCTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAAt                                      <  2:1899596/150‑1 (MQ=255)

                                                                                                                   ggcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAGATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTa              >  1:229954/14‑151 (MQ=255)

                                                                                                                   ggcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTa              >  1:360729/14‑151 (MQ=255)

                                                                                                                    gcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAt             >  2:1751598/13‑151 (MQ=255)

                                                                                                                    gcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAt             >  2:1566576/13‑151 (MQ=255)

                                                                                                                    gcctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTAt             >  2:1566572/13‑151 (MQ=255)

                                                                                                                      ctgccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTc           <  2:1241638/141‑1 (MQ=255)

                                                                                                                        gccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCg          >  2:1118043/9‑150 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCGACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:1630829/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGTCGGCACGTCTTCGcc        <  2:1234103/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  1:1410671/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:614685/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:614491/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  1:1396677/144‑1 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGc         >  1:1105359/8‑150 (MQ=255)

                                                                                                                         ccgccggctaTCTCTTCGGAGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGcc        <  2:1318139/144‑1 (MQ=255)

                                                                                                                             cggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGAc    <  1:47747/148‑1 (MQ=255)

                                                                                                                             cggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGAc    <  1:47749/148‑1 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  1:1316904/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  1:1824504/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  2:1051266/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  1:854107/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGa     >  1:854102/3‑149 (MQ=255)

                                                                                                                              ggctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTCGCCGGCGGCGGCACGTATTCGCCCGa     >  1:1824506/3‑149 (MQ=255)

                                                                                                                               gctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACtt  >  1:689949/2‑151 (MQ=255)

                                                                                                                               gctaTCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACt   <  2:617949/149‑1 (MQ=255)

|||

GGATTACCGGCTTGTCGGCAGCAAAATCCATCCGACCAAATTTACCAATAAAGACGGCAAACCGCGCGCGGTGAAAAAAGCAGATTTGGGCAGATTTGAAGATACGGAGCACCAGTATATGCCGC‑TACTATCTCTTCGGCGAGATTTTCTACGATTCGGATATAGAACATGAAAATGTCGCCAACAACCAAATTGAAGGCGTAATCGTATTTACCAAAATACCGAGAAACTCCATCAAAATCCCTGTTGCCGGCGGCGGCACGTATTCGCCCGACTT  >  Exported/522342‑522618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`