breseq  version 0.37.1  revision d7ab7d0ba1c1
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 55 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,135,7304.G79.4% 113.8 / 53.9 68intergenic (+323/‑69)BCHEFKBB_02184/BCHEFKBB_02185glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
*Exported2,134,6535.G74.4% 78.9 / 38.2 39coding (166/183 nt)BCHEFKBB_02183hypothetical protein
*Exported1,729,7181.G68.3% 191.7 / 105.1 82coding (745/753 nt)BCHEFKBB_01772glycosyl transferase family 1 [NGO_1765]
*Exported2,073,0474.G48.6% 52.9 / 87.3 35intergenic (‑108/‑396)BCHEFKBB_02124/BCHEFKBB_02125FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
*Exported1,887,9430AG34.0% 500.9 / inf 337intergenic (‑326/+255)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported1,887,9340TC33.9% 502.3 / inf 333intergenic (‑317/+264)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported75,3351.G33.3% 21.6 / 17.1 12intergenic (‑76/‑1018)BCHEFKBB_00065/BCHEFKBB_00066Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
*Exported75,3352.A33.3% 19.8 / 15.6 12intergenic (‑76/‑1018)BCHEFKBB_00065/BCHEFKBB_00066Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
*Exported75,3353.G33.3% 21.6 / 14.9 12intergenic (‑76/‑1018)BCHEFKBB_00065/BCHEFKBB_00066Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
*Exported75,3354.A33.3% 19.9 / 12.2 12intergenic (‑76/‑1018)BCHEFKBB_00065/BCHEFKBB_00066Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
*Exported75,3355.C33.3% 22.4 / 14.4 12intergenic (‑76/‑1018)BCHEFKBB_00065/BCHEFKBB_00066Opacity protein opA54 [NGO_11100]/peptidase A8 [NGO_0071]
*Exported1,887,9410CA32.7% 515.3 / inf 337intergenic (‑324/+257)BCHEFKBB_01931/BCHEFKBB_01932GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
*Exported1,831,9900GA31.1% 486.4 / inf 334intergenic (‑123/+230)BCHEFKBB_01881/BCHEFKBB_01882Opacity protein opA54 [NGO_09965]/transposase [NGO_09970]
*Exported1,671,3840CA29.5% 272.7 / 27.8 129Q18K (CAA→AAA) BCHEFKBB_01719ribonuclease II [NGO_1713]
*Exported55,5730TA29.3% 84.9 / 11.3 63H30Q (CAT→CAABCHEFKBB_00050hypothetical protein
*Exported1,572,2120CA28.5% 331.1 / 48.9 194M223I (ATG→ATTBCHEFKBB_01601hypothetical protein [NGO_1599]
*Exported1,202,4050GT27.0% 403.1 / 32.1 215L97F (TTG→TTTBCHEFKBB_01249membrane protein [NGO_1252]
*Exported1,202,4160CA25.8% 398.8 / 10.3 210S101Y (TCC→TAC) BCHEFKBB_01249membrane protein [NGO_1252]
*Exported801,4600CA24.7% 366.7 / 36.2 190R178R (CGC→CGAcysISulfite reductase [NADPH] hemoproteinbeta‑component
*Exported107,0560AT24.7% 355.3 / 50.6 186L44Q (CTG→CAG) BCHEFKBB_00094pilus assembly protein PilP [NGO_0097]

Marginal new junction evidence (lowest skew 10 of 87 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported = 21357206 (0.030)104 (0.430)
+GGGG
28/266 4.7 94.5% intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
?Exported 2135721 = 6 (0.020)intergenic (+314/‑78) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported 2132863 =176 (0.710)79 (0.390)
+TCCCCCCCCCCCCCC
23/244 5.3 47.7% intergenic (+329/‑67) BCHEFKBB_02180/BCHEFKBB_02181 glycyl‑tRNA synthetase subunit beta [NGO_2154]/glycosyl transferase [NGO_11610]
?Exported = 2135720 31 (0.140)intergenic (+313/‑79) BCHEFKBB_02184/BCHEFKBB_02185 glycosyltransferase [NGO_11620]/glycosyl transferase family A [NGO_2158]
* ? Exported = 51928166 (0.670)56 (0.250) 24/270 6.0 21.3% intergenic (‑429/+96) BCHEFKBB_00048/BCHEFKBB_00049 carbamoyl‑phosphate synthase [NGO_0053]/pilus assembly protein [NGO_0055]
?Exported 1887832 = 262 (1.160)intergenic (‑215/+366) BCHEFKBB_01931/BCHEFKBB_01932 GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
* ? Exported = 21346446 (0.030)69 (0.290)
+GGGGG
23/266 6.1 92.0% coding (157/183 nt) BCHEFKBB_02183 hypothetical protein
?Exported 2134645 = 6 (0.020)coding (158/183 nt) BCHEFKBB_02183 hypothetical protein
* ? Exported = 1887130220 (0.890)41 (0.230) 16/210 6.2 19.2% intergenic (+279/+203) BCHEFKBB_01930/BCHEFKBB_01931 membrane protein [NGO_1910]/GIY‑YIG nuclease [NGO_1911]
?Exported 1887862 = 188 (1.070)intergenic (‑245/+336) BCHEFKBB_01931/BCHEFKBB_01932 GIY‑YIG nuclease [NGO_1911]/pilus assembly protein [NGO_10270]
* ? Exported = 260182263 (1.070)40 (0.230)
+42 bp
16/210 6.2 19.2% intergenic (+282/+193) BCHEFKBB_00257/BCHEFKBB_00258 ligand‑gated channel [NGO_1495]/N‑(5'‑phosphoribosyl)anthranilate isomerase[NGO_0261]
?Exported = 1887026 209 (0.850)intergenic (+175/+307) BCHEFKBB_01930/BCHEFKBB_01931 membrane protein [NGO_1910]/GIY‑YIG nuclease [NGO_1911]
* ? Exported 1034985 =208 (0.840)29 (0.330)
+40 bp
4/106 6.4 31.4% intergenic (+401/‑57) BCHEFKBB_01058/BCHEFKBB_01059 hypothetical protein/Opacity protein opA54 [NGO_05620]
?Exported = 1231240 93 (0.600)intergenic (+681/‑103) BCHEFKBB_01273/BCHEFKBB_01274 hypothetical protein [NGO_1277]/Opacity protein opA54 [NGO_06725]
* ? Exported = 925782245 (0.990)53 (0.330) 11/190 7.4 28.4% intergenic (+275/‑78) BCHEFKBB_00943/BCHEFKBB_00944 Opacity protein opA54 [NGO_08230]/TonB‑dependent receptor [NGO_0952]
?Exported 1036177 = 109 (0.690)intergenic (+446/‑345) BCHEFKBB_01059/BCHEFKBB_01060 Opacity protein opA54 [NGO_05620]/acyl‑CoA thioester hydrolase [NGO_1078]
* ? Exported = 207303822 (0.100)48 (0.200)
+GGGG
19/268 7.6 68.5% intergenic (‑99/‑405) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
?Exported 2073039 = 22 (0.090)intergenic (‑100/‑404) BCHEFKBB_02124/BCHEFKBB_02125 FetA [NGO_2093]/molecular chaperone GroES [NGO_2094]
* ? Exported = 1231240NA (NA)77 (0.390)
+30 bp
4/126 7.8 NA intergenic (+681/‑103) BCHEFKBB_01273/BCHEFKBB_01274 hypothetical protein [NGO_1277]/Opacity protein opA54 [NGO_06725]
?Exported 1532311 = NA (NA)intergenic (+293/‑67) BCHEFKBB_01558/BCHEFKBB_01559 proline dehydrogenase [NGO_08225]/Opacity protein opA54 [NGO_08230]