Data for: TRIM21 post-translationally regulates cytoplasmic APOBEC3 enzymes

Description

This file contains all raw data reported in the manuscript, organized by figure.

Each subfolder contains raw Western blotting images, Empiria studio Western blot image analyses with points quantified highlighted, raw infectivity values and calculations, raw qPCR data and calculations, and raw p24 values with calculations. 

Western blot file titles indicate date run, cell type, loading order, and antibody probe used. 

Empiria Studio analysis files show images analyzed, bands analyzed, and resulting signal values.

Western blot calculation files contain loading control and target protein signal values.

qPCR files contain CT values for internal control (18s) and gene targets in triplicate. qPCR calculation files show resulting RQ values obtained from 2^-ΔΔCT analysis.

P24 files show average pg/mL values, standard deviation, and coefficient of variance for each sample. Calculations are for % change in p24 production.

Infectivity files show plate layouts and raw luciferase signal for each sample in triplicate. Calculations show infectivity relative to control normalized to 100%.