Evaluating the interplay between estrous cyclicity and flurothyl-induced seizure susceptibility in Scn2aK1422E mice - Supplementary Table S1

Description

Evaluating the interplay between estrous cyclicity and induced seizure susceptibility in Scn2a K1422E mice

Supplementary Table S1: Estrous cycle monitoring data

Contents:

Sheet 1: Estrous cycle - OVX, sham

- Detailed estrous cycle monitoring data for all subjects that underwent OVX or sham surgery

- Subjects are identified by study number in column A

- Genotype information (WT, E/+) is given in column B

- Surgical condition (OVX, sham) is given in column C

- Estrous cycle stage calls (see legend, column W) are given in columns D-R

- Cycle regularity call (regular, irregular) is given in column S

- Blinded call of surgical condition (OVX, Sham) is given in column T

- Latency to flurothyl-induced GTCS is given in column U

-Total days monitored is given in column W (sum of columns X,Y,Z,AA)

-Number of monitoring days in diestrus is given in column X

-Number of monitoring days in proestrus is given in column Y

-Number of monitoring days in estrus is given in column Z

-Number of monitoring days in metestrus is given in column AA

-Number of monitoring days in unclear and/or with few cells is given in column AB

-Percent of monitoring days in diestrus is given in column AD (column X divided by column W *100%)

-Percent of monitoring days in proestrus is given in column AE (column Y divided by column W *100%)

-Percent of monitoring days in estrus is given in column AF (column Z divided by column W *100%)

-Percent of monitoring days in metestrus is given in column AG (column AA divided by column W *100%)

***rows in red indicate subjects where blinded call of surgical condition did not match actual condition***

Sheet 2: Estrous cycle - untreated

- Detailed estrous cycle monitoring data for all non-surgerized subjects 

- Subjects are identified by study number in column A

- Genotype information (WT, E/+) is given in column B

- Estrous cycle stage calls (see legend, column R) are given in columns C-N

- Cycle regularity call (regular, irregular) is given in column O

- Latency to flurothyl-induced GTCS is given in column P

-Total days monitored is given in column R (sum of columns S,T,U,V)

-Number of monitoring days in diestrus is given in column S

-Number of monitoring days in proestrus is given in column T

-Number of monitoring days in estrus is given in column U

-Number of monitoring days in metestrus is given in column V

-Percent of monitoring days in diestrus is given in column X (column S divided by column R *100%)

-Percent of monitoring days in proestrus is given in column AE (column T divided by column R *100%)

-Percent of monitoring days in estrus is given in column AF (column U divided by column R *100%)

-Percent of monitoring days in metestrus is given in column AG (column V divided by column R *100%)

Additional details